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猪大肠杆菌和葡萄球菌的分离鉴定及耐药性分析 被引量:11

Isolation,Identification and Analysis of Drug Resistance on E. coli and Staphylococcus in Pigs
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摘要 对湘西北地区某猪场患病的仔猪的粪便及病变的脾脏、肝脏、淋巴结、肺脏、肾水肿液进行病原菌分离鉴定,并用Kirby-Bauer法测定分离菌株对15种抗菌药的敏感性。结果表明,患病仔猪分离到大肠杆菌和猪葡萄球菌两种细菌。其中大肠杆菌对庆大霉素、头孢呋肟、丁胺卡那霉素、四环素、先锋必素敏感,对氯霉素、氧哌嗪青霉素、先锋五号、头孢曲松钠、新霉素中介,对羧苄青霉素、强力霉素、青霉素、氨苄青霉素、红霉素有较高的耐药性。葡萄球菌对先锋霉素Ⅴ、丁胺卡那霉素敏感,对头孢曲松钠中介,对其他抗生素耐药。其中大肠杆菌对药物敏感率、中介率、耐药率均为33.33%;葡萄球菌的敏感率为13.33%,中介率为6.67%,耐药率为80.00%。 The pathogen were isolated and identified on feces of sick piglets and disease of the spleen,liver,lymph nodes,lung,kidney edema fluidin a pig farm in Hunan northwestern region.Susceptibility of 15 kinds antimicrobial drug was determined with the Kirby-Barer for isolated strains.The results showed piglets were infected with E.coli-based and Staphylococcus.Escherichia coli was sensitive to gentamicin,cephalosporins latter skin,Amikacin,tetracycline,cefoperazone sodium,to chloramphenicol,piperacillin,pioneer V,ceftriaxone,neomycin was intermediate,to carbenicillin,doxycycline,penicillin,ampicillin,erythromycin had a higher resistance.Staphylococcus was sensitive to pioneer V,Amikacin,to ceftriaxone was intermediate,on the other antibiotic was resistance.Which was 33.33% the rate of drug sensitivity,intermediate and resistance to E.coli.Which was 13.33% sensitive rate,intermediate rate was 6.67%,resistance rate was 80.00% to Staphylococcus.
出处 《中国畜牧兽医》 CAS 北大核心 2012年第8期195-198,共4页 China Animal Husbandry & Veterinary Medicine
基金 湖南省动物学重点实验室资助(2008) 湖南省高校创新平台开放基金项目(10K043) 湖南省"十二五"重点建设学科(动物学)项目(2011)
关键词 大肠杆菌 葡萄球菌 药敏试验 pig Escherichia coli Staphylococcus drug susceptibility testing
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