摘要
目的探讨初培养产物结合PCR方法检测精液解脲脲原体感染应用价值。方法选取120例不育症患者的精液,分别用PCR、培养加药敏试剂盒、初培养产物结合PCR的方法检测解脲脲原体,并与分离培养结果相比较。结果120例精液分离培养出62例解脲脲原体,使用PCR法检测出30例阳性、用培养加药敏试剂盒检测出65例阳性、用初培养产物结合PCR技术检测出62例阳性,以上三种方法与分离培养结果的总符合率分别为73.3%、97.5%和100%。结论初培养产物结合PCR是一种检测精液解脲脲原体的可行性方法。
Objective To explore the applied value of using initial culture binding polymerase chain reac- tion （PCR） assay for the detection of infection of Ureaplasma urealyticum in semen. Methods The semen sam- pies were collected from 120 sufferers with sterility, Ureaplasma urealyticum were detected by 3 methods： PCR, culture, initial culture binding PCR, respectively, and the results were anglyzed. Results Of the 120 samples, Ureaplasma urealyticum were isolated in 62 cases by isolation and culture, positive in 30 cases by PCR, posi- tive in 65 cases by culture and drug susceptibility kit, and positive in 62 cases by initial culture binding PCR. Compared with isolating culture method, the coincidence was 73.3%, 97.5% and 100% respectively. Conclusion Initial culture binding PCR is a kind of feasible means to detect Ureaplasma urealyticum in semen.
出处
《实验与检验医学》
CAS
2012年第4期361-363,共3页
Experimental and Laboratory Medicine
关键词
解脲脲原体
PCR
培养
精液
Ureaplasma urealyticum
Polymerase chain reaction
Culture
Semen
