摘要
Objective To characterize microRNA （miRNA） expression profile in microdissected auditory epithelia from the Corti＇ s Organ in new born and adult rats. Methods The TaqMan（R） MicroRNA Arrays were used to identify expres- sion of microRNA in the new born and adult groups. GO analysis was applied to analyze the main function of the differen- tial expression genes according to the Gene Ontology which is the key functional classification of NCBI. Similarly, Pathway analysis was used to find out the significant pathway of the differential genes according to KEGG, Biocarta and Reatome. Results Increased expression was seen in 16 miRNAs in mature rat compared to new born rats, with increased folding ranging from 17 to 600 folds. Expression levels in 2 miRNAs were reduced in mature rats, namely rno-miR-29c and rno-miR-29a. The high-enrichment GOs targeted by over-expressed miRNAs were negative regulation of epithelial cell differentiation, common-partner SMAD protein phosphorylation, mesenchymal-epithelial cell signaling, regulation of transforming growth factor beta2 production, etc. Functional analysis of miRNAs by KEGG revealed that 19 signal transduction pathways were upregulated and 14 were downregulated. Conclusions The difference in miRNA expression patterns in the organ of Corti between neonatal and adult rats may be closely related to maturation of the organ of Corti and loss of proliferative capacity of inner ear hair cells, and TGFβ signaling may play an important role in hair cells regeneration.
Objective To characterize microRNA （miRNA） expression profile in microdissected auditory epithelia from the Corti＇ s Organ in new born and adult rats. Methods The TaqMan（R） MicroRNA Arrays were used to identify expres- sion of microRNA in the new born and adult groups. GO analysis was applied to analyze the main function of the differen- tial expression genes according to the Gene Ontology which is the key functional classification of NCBI. Similarly, Pathway analysis was used to find out the significant pathway of the differential genes according to KEGG, Biocarta and Reatome. Results Increased expression was seen in 16 miRNAs in mature rat compared to new born rats, with increased folding ranging from 17 to 600 folds. Expression levels in 2 miRNAs were reduced in mature rats, namely rno-miR-29c and rno-miR-29a. The high-enrichment GOs targeted by over-expressed miRNAs were negative regulation of epithelial cell differentiation, common-partner SMAD protein phosphorylation, mesenchymal-epithelial cell signaling, regulation of transforming growth factor beta2 production, etc. Functional analysis of miRNAs by KEGG revealed that 19 signal transduction pathways were upregulated and 14 were downregulated. Conclusions The difference in miRNA expression patterns in the organ of Corti between neonatal and adult rats may be closely related to maturation of the organ of Corti and loss of proliferative capacity of inner ear hair cells, and TGFβ signaling may play an important role in hair cells regeneration.
基金
supported by grants from the Major State Basic Research Development Program of China(973 Program)(#2011CBA01000)
the National Basic Research Program of China(973 Program)(#2012CB967900)
the National Natural Science Foundation of China(NSFC)(#81000483)
New Star Project of Science and Technology of Beijing(2010B083)to Z.H.H
