摘要
目的:建立槲寄生中紫丁香苷的高效液相含量测定方法,为药材及其制剂的质量控制提供依据。方法:色谱柱:AgilentExtend C_(18)柱(250 nn×4.6 nn,5μm);流动相:甲醇-0.1%磷酸溶液(15:85);检测波长:264 nn。结果:紫丁香苷在0.25～4.98μg范围内线性关系良好,r=0.999 9,平均加样回收率为102.5%,RSD=1.6%(n=9)。结论:该方法简便、快速、准确、重复性好,为槲寄生药材及其制剂的质量控制提供了依据。
Objective： To establish an HPLC method for the quantitative determination of syringin in Viscum coloratum （ Komar. ） Nakai to control the quality. Method： An Agilent Extend C18 column （250 mm ×4.6 mm,5 μm）, a mobile phase of methanol-water containing 0.1% phosphoric acid （ 15：85 ） and the detection wavelength of 264 nm were applied in the study. Result： The calibration curve was in good linearity over the range of 0.254.98 μg（ r = 0.999 9）. The average recovery of syringin was 102.5% with RSD of 1.6% （n = 9 ）. Conclusion： The method is simple, sensitive, accurate and reproducible both for the quality control of the crude material and preparation of Viscum coloratum （Komar.） Nakai.
出处
《中国药师》
CAS
2012年第7期971-973,共3页
China Pharmacist
基金
<中国药典>2010年版一部标准研究课题(编号:YS-107
YS-108)
