摘要
目的:建立舞草中木犀草素的含量测定方法。方法:采用VP-ODS C18(4.6 mm×250 mm,5μm)色谱柱。以甲醇-0.3%磷酸溶液(60∶40)为流动相,流速1.0 mL.min-1,检测波长350 nm。结果:舞草中的木犀草素含量测定方法在0.0407 2～0.6515 2进样量与峰面积的线性关系良好(r=0.999 8);平均回收率100.35%,RSD 0.80%。结论:本方法准确、灵敏、重复性好,可用于舞草的质量控制。
Objective： To establish the determination method of luteolin content in Codariocalyx motorius. Method： VP-ODS C18 （4.6mm×250mm,5μm） colmnn was used, the mobile phase was methanol-0.3% phosphonic acid solution nm. Result： The linear recovery was 100.35% , in reproducibility. It can （60： 40） with the flow rate as 1.0 mL ·min -1 The detection wavelength was set at 350 relation of hyperin was good in the range from 0. 040 72 to 0. 651 52 μg. The average RSD was 0. 80%. Conclusion： The method established is accurate, sensitive and good be applied to the quality control of C. motorius.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第17期145-147,共3页
Chinese Journal of Experimental Traditional Medical Formulae
