摘要
目的:研究二氢杨梅素(DMY)对复发性口腔溃疡(RAU)大鼠肿瘤坏死因子(TNF-α)及NF-κB p65的影响。方法:将60只SD大鼠随机分为6组,采用同种异体口腔黏膜匀浆作为抗原皮内注射5次,制备RAU大鼠模型。末次免疫注射的同时开始给药,正常对照组和模型组给予蒸馏水,其他组分别给DMY 50,100,200 mg.kg-1和甘草锌67.5 mg.kg-1,按10mL.kg-1ig给药7 d。取血分离血清;取口腔黏膜制备匀浆。ELISA测定口腔黏膜和血清中TNF-α的含量;免疫组化法和天青-伊红-瑞氏染色法分别检测口腔黏膜中NF-κB p65,巨噬细胞(MΦ)的表达;逆转录聚合酶链式反应法(RT-PCR)检测口腔黏膜和血清中TNF-αmRNA的表达。结果:RAU模型大鼠血清和口腔黏膜中TNF-α含量显著升高,TNF-αmRNA表达明显增强,口腔黏膜组织中NF-κB p65,MΦ表达增加;DMY能降低口腔黏膜组织和血清中TNF-α含量和TNF-αmRNA的表达,降低口腔黏膜中NF-κB p65,MΦ表达。结论:DMY可能通过抑制RAU口腔黏膜组织中NF-κB p65对MΦ中TNF-αmRNA的转录和释放的调节作用,使口腔黏膜中TNF-α下降而起抗口腔溃疡的作用。
Objective： To study the effects of dihydromyricetin（DMY） on tumor necrosis factor（TNF-α） and NF-κB p65 cells of the recurrent aphthous ulcer（RAU） rat. Method： Sixty of Sprague Dawley（SD） rats are randomly divided into 6 groups. The rat RAU models was established by injection of immunogen composed of the homogenate supernate of homogeneousoral oral mucosa from SD rats and Freund＇s complete adjuvant（FCA） into rat backs subcutaneously once every two weeks for 5 times,and the only FCA injected as normal control. DMY（50,100,200 mg·kg-1） and licorzine （67.5 mg·kg-1） were given intragastrically once daily for 7 days on the day of the last immunogen injection,respectively. Water was given instead of drugs in normal and model control groups. The blood was got from the fundus oculi vein of rats on the day after last administration,the serum was separated. Then the rats were put to death with the cervical dislocation and decollated on the ice stage. Two sides of rat buccal mucosal tissue were cut. One side of them was put into 4% neutral formalin and another was added into 10 times of phosphate buffer to homogenize it homogenate. The oral mucosa ulcer occurrence of rats was observed by the histopathology.The content of TNF-α in serum and oral mucosa was assayed with ELISA; the expression of NF-κB cells was determined by the immunohistochemisty and macrophagus was determined by azure-feosin-dyeing in oral mucosa tissue. The expression of TNF-α mRNA in serum and oral mucosa was detected by reverse transcription polymerase chain reaction. Result： In RAU rats,oral mucosa ulcer occurred,the content of TNF-α raised and the expression of TNF-α mRNA increased in serum and oral mucosa, the expression of positive NF-κB p65 cells and the amount of macrophages went up in oral mucosa.DMY and licorzine significantly reduced occurrence of oral mucosa ulcer in RAU rats, lowered content of TNF-α and the expression of TNF-α mRNA in serum and oral mucosa, reduced expression of positive NF-κB p6
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第17期2612-2617,共6页
China Journal of Chinese Materia Medica
基金
湖南省三项基金项目(04SK3067)
