摘要
目的以高脂饲料饲养的SPF级C57 BL/6 ApoE-/-小鼠作为动物模型,研究茶多酚采食对其肠道菌群多样性的影响。方法通过随机饮水的方式给予ApoE-/-小鼠0.4、0.8和1.6 g/L的茶多酚,处理14 d时用PCR-DGGE分析对照组(CK)和茶多酚组小鼠新鲜粪便中肠道菌群的相似性和多样性。结果 UPGMA聚类分析表明,低剂量茶多酚组(LTP)、中剂量茶多酚组(MTP)这两组与对照组(CK)、高剂量茶多酚组(HTP)聚为两大簇。PCA分析显示,LTP组与CK组、MTP组、HTP组分别聚集在不同位置,有明显界限;多样性数据分析显示:CK组DGGE图谱的丰富度和Shannon-Wiener指数(H')与茶多酚组差异无统计学意义(P>0.05);CK组与MTP组的均匀度(E)差异存在统计学意义(P<0.05,P=0.015),说明中剂量茶多酚作用14 d后与对照组肠道菌群的菌群分配相比均一性显著下降。结论连续处理14 d时CK组与LTP组、MTP组小鼠肠道菌群差异有统计学意义,即茶多酚对ApoE-/-小鼠肠道菌群多样性有显著影响。
Objective To study the effects of green tea polyphenols （GTP） on the diversity of intestinal microflora molecular profile in mice. Methods SPF ApoE-/- mice were fed with high-fat diet and drinking water containing 1.6 g/L, 0.8 g/L and 0.4g/L GTP respectively for 14 days. Fecal samples were collected during the period of the mice feeding with GTP. Diversity of intestinal microbiota in feces was studied with PCR-DGGE method. Results Similarity cluster analysis of DGGE profile showed that low-dose GTP group and medium-dose GTP group were clustered into two major clusters with control group and high-dose GTP group. Principal component analysis indicated that the low-dose GTP group and control group, medi- um-dose GTP group and high-dose GTP group were located in different regions respectively. No remarkable differences were observed in the richness, Shannon-Wiener indexes of intestinal microflora between the control and GTP groups by statistical analysis （ P 〉 0.05 ） ; Significant differences were observed in the evenness degree of intestinal mieroflora between control group and medium-dose GTP group （ P 〈 0. 05, P = 0.015 ）. Conclusion Tea polyphenols can affect the diversity of mice intestinal mieroflora significantly.
出处
《中国微生态学杂志》
CAS
CSCD
2012年第8期673-676,共4页
Chinese Journal of Microecology
基金
国家自然科学基金(31071528)
