摘要
目的 探讨鸟苷素在哮喘豚鼠发病中的变化。方法 16只豚鼠随机分为两组 :对照组(6只 )和哮喘组 (10只 ) ,采用放射免疫法测定血浆、支气管肺泡灌洗液 (BALF)及肺组织鸟苷素 ,图像分析软件测定气道壁厚度及腺体厚度。结果 哮喘组动物血浆及肺组织鸟苷素分别较对照组增加5 6 %及 183% ,差异有显著性 (P <0 0 1)。对照组BALF鸟苷素 4只低于检测水平 ,另 2只分别为 1 7pg/ml及 1 6pg/ml,哮喘组BALF鸟苷素为 (3 1± 1 2 )pg/ml。肺组织鸟苷素水平分别与血浆鸟苷素水平及BALF鸟苷素水平呈正相关 (r分别 =0 72、0 6 1,P <0 0 1、<0 0 5 ) ;血浆鸟苷素水平与BALF鸟苷素水平无明显相关 (r=0 32 ,P >0 0 5 )。图像分析结果表明 ,哮喘组气道壁厚度及腺体厚度分别较对照组增加 86 %和 87% ,差异有显著性 (P <0 0 1) ,肺组织鸟苷素水平与腺体厚度呈正相关 (r =0 95 ,P <0 0 1)。结论 鸟苷素可能参与哮喘豚鼠气道分泌增加的发病机制。
Objective To study the changes of guanylin in asthmatic guinea pig models Methods Male guinea pigs were randomly divided into two groups: the control ( n =6) and the asthma group ( n =10) The levels of guanylin in plasma, BALF and lung tissue were measured by RIA kit The mean thickness of airway wall and glands was determined by contour tracing, using a digitizing pad and microcomputer Results The levels of guanylin in plasma and lung tissue from the asthma group were 56% and 183% higher than those of the control ( P <0 01) Concentrations of guanylin in BALF from the control were below the detection level in 4 guinea pigs, and 1 7 pg/ml and 1 6 pg/ml in two,respectively The level of guanylin in BALF from asthma group was ( 3 1±1 2)pg/ml The level of guanylin in the lung tissue correlated with that in plasma and BALF ( r = 0 72, P <0 01, r =0 61, P <0 05, respectively) The level of guanylin in plasma did not correlate with those of BALF ( r =0 32, P >0 05) The mean thickness of airway wall and glands in asthma group were 86% and 87% greater than those of the control respectively ( P <0 01) The level of guanylin in lung tissue correlated with the thickness of glands ( r =0 95, P <0 01) Conclusions In the guinea pig model of asthma, guanylin in plasma , BALF and lung tissue were increased, indicating that it may play a role in the mechanism of airway hypersecretion in asthma
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2000年第7期410-412,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
