摘要
目的探讨轻度认知障碍患者血小板中α、β-分泌酶基因表达水平及其临床意义。方法选入28例轻度认知障碍(MCI)患者,以28例老年性痴呆(AD)及21例正常老人为对照,分别用实时荧光定量PCR检测各组血小板中的ADAM10、BACE1基因表达水平,用ELISA法检测各组血浆中Aβ42含量。结果 3组中β-分泌酶(BACE1)基因表达水平有明显差异(P<0.05),AD组、MCI组的表达水平是正常老人的4.51倍、2.22倍。α分泌酶基因表达水平及Aβ42含量无明显差异(P>0.05),相关分析显示Aβ42含量与α、β-分泌酶基因表达水平无关(r=-0.1442,r=0.1611,P均>0.05)。结论 MCI患者血小板中的BACE1的基因表达上调可能与MCI的转向(向AD转化)有关。MCI患者应尽早接受相关的酶学检测并实施干预。
Objective To detect the expression of ADAM10 and BACE1 mRNA in mild cognitive im- pairment and explore the roles of ADAM10 and BACEI in the development of MCI. Methods 28 cases with MCI,28 cases with AD and 21 cases normal elders were selected. Real-time fluorescence quantitative RT-PCR was performed for detecting the expression of ADAM10 and BACE1 mRNA, A sandwich ELISA was per- formed for detecting the blood serum Aβ42 concentration. Results The FQ-PCR results showed that the expres- sion level of Platelet BACE1 mRNA in AD,MCI groups were 4. 51-fold and 2. 22-fold higher than the normal elders respectively（P〈0. 05）. The expression level of Platelet ADAM10 mRNA and serum Aβ42 concentration showed no differences（r = - 0. 1442, r = 0. 1611, P2〉0. 05）. Serum Aβ2 concentration showed no correlations with expression levels of Platelet ADAM10 BACE1 mRNA respectively. Conclusions Platelet BACE1 mRNA expression might be associated with the development MCI and may potentially used as an indicator for predic- ting the prognosis of MCI.
出处
《卒中与神经疾病》
2012年第4期195-198,206,共5页
Stroke and Nervous Diseases
基金
国家自然基金资金项目(30870863)
广东省医学科研基金项目(A2006003)
关键词
轻度认知障碍
分泌酶
PCR
Mild cognitive impairment
Secretase
Real-time fluorescence quantitative （RT-PCR）
