摘要
目的研究Cpn0147重组蛋白的免疫学活性及其应用于肺炎嗜衣原体(Cpn)感染临床诊断中的价值。方法采用GST琼脂糖凝胶纯化目的蛋白,将Cpn0147重组蛋白免疫新西兰兔,分别应用Westernblot、ELISA法检测其免疫反应性及免疫原性,同时通过检测临床标本以评估其诊断价值。结果成功表达并纯化了相对分子质量约41×103的重组蛋白GST-Cpn0147;Westernblot和ELISA结果显示,该重组蛋白具有良好的免疫反应性,动物实验结果表明该重组蛋白具有较好的免疫原性,ELISA结果显示该重组蛋白免疫新西兰兔血清抗体效价为1:12800。结论 Cpn0147重组蛋白具有较好免疫学活性和特异性,可为Cpn感染的确诊、预防研究奠定基础。
Objective To explore the immunoactivity of Cpn 0147 recombinant protein,and its clinical value for the diagnosis of Chlamydophila pneumoniae（Cpn） infection.Methods GST-Cpn 0147 protein was purified by GST sepharose FF.Its immunoactivity was determined by Western blot,and clinical specimens were detected by enzyme linked immunosorbent assay（ELISA）.Results The GST fusion protein with molecular weight of 41×103 was expressed.The purity of recombination protein after purification reached 95%.Both Western blot and ELISA showed good antigenicity of recombination protein.Conclusion The purified recombination protein might have high specificity and good immunological activity,which could lay a solid foundation for the further study on the serodiagnosis and prevention of Cpn.
出处
《国际检验医学杂志》
CAS
2012年第14期1671-1672,1674,共3页
International Journal of Laboratory Medicine
基金
湖南省科技厅科研基金项目(2011SK3059)
关键词
衣原体
肺炎
Cpn0147重组蛋白
临床诊断
Chlamydophila pneumoniae； Cpn 0147 recombinant protein； clinic diagnosis
