摘要
目的建立同时分离检测麻黄中麻黄碱(Ephedrine)、伪麻黄碱(Pseudoephedrine)、去甲麻黄碱(Norephedrine)、去甲伪麻黄碱(Norpseudoephedrine)和甲基麻黄碱(Methylephedrine)的含量测定方法。方法采用HPLC-UV法,色谱柱:苯基柱(Alltima Phenyl,250mm×4.6 mm,5μm);流动相:0.02 mol/L磷酸二氢钾溶液-乙腈(96∶4);流速:0.6 ml/min;检测波长:210 nm;柱温:25℃。结果 5种生物碱在13.5 min内即可达到完全分离,E在2.000 8～40.016 0μg/ml线性关系良好;PE在1.003 6～20.072 0μg/ml线性关系良好;NME在0.199 2～3.984 0μg/ml线性关系良好;NMP在0.200 0～4.00 0μg/ml线性关系良好;ME在0.200 4～4.008 0μg/ml线性关系良好。各生物碱的平均回收率均在92%～104%(RSD≤5.76%)。结论本方法可操作性强,简便快速,分离效果好,重现性好,可为麻黄及含麻黄的中西药制剂提供有效的检测手段。
Objective To establish HPLC-UV method to simultaneously determine the contents of ephedrine, pseudoephedrine, norephedrine, norpseudoephedrine, and methylephedrine in Ephedrae Herba. Methods Alhima Phenyl column （250 mm ×4.6 mm, 5 um） maintained at 25 ℃ with 0.02 mol/L KH2PO4-acetonitrile （96： 4） as the mobile phase was used. The flow rate was 0.6 ml/min and the detective wavelength was at 210 nm. Results The 5 ephedrine alkaloids were separated in 13.5 min. The calibration curve for ephedrine, pseudoephedrine, norephedrine, norpseudoephedrine, and methylephedrine was linear in the range of 2. 000 8 - 40.016 0 ug/ml, 1. 003 6 - 20. 072 0 ug/ml,0. 199 2 - 3. 984 0 ug/ml,0.200 0 -4. 000 0 ug/ml and 0. 200 4 -4. 008 0 ug/ml, respectively. The average recovery was among 92% - 104% （RSD ≤5.76% ）. Conclusion This method shows excellent operability, separation, linearity, specificity, accuracy, precision. It is suitable for the separation and quantization of ephedrines in finished product containing Ephedra sinica or ephedrine alkaloids.
出处
《今日药学》
CAS
2012年第7期388-391,397,共5页
Pharmacy Today
基金
国家自然科学基金资助项目(编号:30772756)
