摘要
采用ITS PCR-RFLP方法对供试116株木霉属菌株进行多样性分析,对其中22株抗病优良菌株进行抗病多样性差异分析,用NTSYS-PC生物软件对DNA条带进行聚类分析,结果显示,HaeIII酶切共获得11个条带,可将供试菌株分为12种基因型;Hinf I共获得13个条带,可将供试菌株分为15种基因型。由116株木霉菌株的聚类图表明,木霉属菌株遗传相似系数变异范围为0.72-1.00。由22株抗病优良菌株的聚类图表明,在遗传相似系数为0.66处可将22株菌分为2类,其中ACCC 30371由于其酶切图谱的特异性在遗传相似系数为0.66处同其他菌株完全分开;在遗传相似系数为0.73处可将剩余21株菌分为2类,第一类中对9种病原菌全部表现抗性的菌株有6株,第二类中对9种病原菌全部表现抗性的菌株有2株。结果证明,本研究方法基本可将木霉属真菌归类,但所获数据在分析本属的抗病差异性这一现象上不够充分,对于其中可能存在的原因予以分析探讨。
ITS-RFLP analysis was used to investigate the genetic diversity of 116 Trichoderma strains and the disease resistance diversity of the 22 Trichoderma strains with strong resistance, the dendrogram was generated by Unweighed Pair Group Method with Arithmetic Mean （ UPGMA ） method with NTSYS-PC biological software. There are 11 and 13 restriction fragments obtained by digestion of Hae III and Hinf I, respectively. While there are 11 restriction fragments and 12 genotypes by the use of Hae III, 13 restriction fragments and 15 genotypes by the use of Hinf I, the difference of the latter is higher. The similarity coefficients of 116 Trichoderma strains ranged from 0.72 to 1.00. The similarity coefficients of 22 Trichoderma strains of fine disease resistance showed that, 22 strains were clustered into 2 groups at the similarity coefficient of 0.66. Because of its special restriction map, ACCC 30371 can be completely separated from other 21 strains at the similarity coefficient of 0.66. The rest 21 strains were clustered into 2 groups at the similarity coefficient of 0.73. In the first group, there were 6 strains which have full resistance of 9 pathogens, the second group have only 2 such strains. The results indicated that this method can be used to classify Trichoderma strains, but the current result is not a sufficient evidence to explain the phenomenon of differences in diversity and disease resistance of Trichoderma. There are some discussions about the probable reasons of this phenomenon.
出处
《生物技术通报》
CAS
CSCD
北大核心
2012年第8期146-153,共8页
Biotechnology Bulletin
关键词
木霉属
ITS-RFLP
内切酶
抗病性
多样性
Trichoderma
ITS-RFLP
Restriction endonuclease
Disease resistance
Diversity
