摘要
目的探讨多西他赛对体外培养的人子宫内膜腺癌Ishikawa细胞凋亡的诱导作用。方法体外培养Ishikawa细胞,不同浓度的多西他赛作用48 h后,用MTT法和流式细胞术检测多西他赛对Ishikawa细胞增殖和细胞周期的影响。结果多西他赛对Ishikawa细胞的增殖具有明显抑制作用,呈剂量依赖性（P〈0.05）。多西他赛引起细胞阻滞在G1~S期,细胞凋亡率增加,呈剂量依赖性（P〈0.05）。结论人子宫内膜腺癌Ishikawa细胞对多西他赛具有药物敏感性,多西他赛对人子宫内膜腺癌Ishikawa细胞的杀伤作用是通过诱导细胞凋亡途径实现的。
Objective To investigate the in vitro effects of docetaxel on proliferation and apoptosis of cultured human endometrial adenocar- cinoma Ishikawa cells. Methods Human endometrial adenocarcinoma Ishikawa cells were cultured in vitro and treated with different con- cenlxation of doeetaxel for 48 h respectively ; MTT assay and flow cytometry were used to measure the effects of docetaxel on cell proliferation and cell cycle. Results Docetaxel inhibited cell proliferation of cultured Ishikawa cells in a dose dependent manner （P 〈 0.05 ）. Docetaxel blocked the cell cycle in G1-S period and increased the rates of cell apoptosis in a dose dependent manner （P 〈 0.05 ）. Conclusion Doc- etaxel is effective for inhibiting the growth of endometrial adenocarcinoma Ishikawa cells. This anti-neoplastic effect may function through cell cycle arrest in G1-S period and increased cell apoptosis.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2012年第8期716-718,共3页
Journal of China Medical University
基金
沈阳市科技局资助项目(F10-205-1-58)
