摘要
为获得可用于体细胞核移植中需要的供核细胞,利用组织块法培养莆田黑猪猪耳皮肤成纤维细胞,并对莆田黑猪猪耳皮肤成纤维细胞进行传代及冷冻。通过试验,获得能在体外正常传代培养的细胞系,为莆田黑猪克隆研究提供供核细胞来源。
In order to get donor cells for somatic cells nuclear transfer , Tissue piece culture dissociate ear skin fibroblast in Putian black pigs in this study, and transfer of culture and freezing.Through the study, We gained cell lines that can normally serial subeuhivation in vitro which offer cell resources for Putian black pig clone research.
出处
《福建畜牧兽医》
2012年第4期9-11,共3页
Fujian Journal of Animal Husbandry and Veterinary medicine
基金
福建省科技计划项目(2008N0001)资助
关键词
莆田黑猪
成纤维细胞
体外培养
冷冻
Putian black pig Fibroblast in vitro culture Freezing
