摘要
目的:研究舍格伦综合征(SS)患者血清中骨桥蛋白(OPN)等细胞因子的含量及相互关系。方法:经病理学检查确诊的SS患者40例,其中原发性15型,继发性25例。酶联免疫吸附试验(ELISA)检测患者血清中骨桥蛋白,肿瘤坏死因子-α(TNF-α),转化生长因子-β1(TGF-β1)的含量。以20名健康志愿者作为正常对照。结果:与正常对照组相比,OPN和TNF-α水平在SS患者显著增加(P<0.05);继发性SS的TGF-β1血清水平显著高于原发性SS,但OPN和TNF-α的血清水平在原发性SS与继发性SS中没有显著差异(P>0.05)。结论:骨桥蛋白在原发性SS和继发性SS患者的血清中表达均高于正常对照组,但继发性SS较原发性SS表达更为显著。说明其表达与疾病的严重程度有关。继发性SS的TGF-β1血清水平显著高于原发性SS,说明继发性SS疾病的病理发生发展更为复杂。
Objective： To explore the relationship of serum concentration of＇osteopontin （OPN） and other proinflam- matory cytokines in Sjogren＇s syndrome （SS） patients. Method： twenty-six patients with pathologically-confirmed SS （pri- mary SS, n=15; secondary SS, n=25） were investigated. Serum levels of OPN, TNF-alpba and TGF-beta 1 were examined by enzyme linked immunosorbent assay （ELISA）. Twenty healthy subjects were served as normal controls. Result： Serum OPN, TNF-alpha levels were increased in SjSgren＇s syndrome patients in comparison with healthy subjects （P 〈0.05）. TGF-betal levels in secondary SS were increased remarkedly than that in primary SS in serum （P 〈0.05）. No statistically significant dif- ference was found in the prevalence of OPN and TNF-alpha expression between the two kinds of SS （P 〉0.05）. Conclusion： Serum OPN levels were increased in both primary SS patients and secondary SS, and secondary SS OPN expression more re- mark than primary SS. This suggested that OPN expression was correlated with the progression of disease. TGF-betal levels in secondary SS were increased than that in primary SS, indicating that the pathogenesis was more complicated in secondary SS.
出处
《临床口腔医学杂志》
2012年第8期462-464,共3页
Journal of Clinical Stomatology
基金
上海高校选拔培养优秀青年教师专项基金(JDY08036)
