摘要
目的研究医院医护人员携带耐甲氧西林金黄色葡萄球菌及其抗药基因携带情况,为抗菌药物合理使用提供参考。方法采用细菌分离鉴定方法和基因扩增技术,对医院医护人员鼻前庭和手上采集的标本进行了细菌分离鉴定和抗药基因检测。结果上海市医院4种岗位医护人员鼻前庭MRSA平均携带率为1.81%,手上MRSA平均携带率为0.83%,以医生和工勤人员携带率较高。鼻前庭分离的MRSA菌株qacA基因阳性率为33.33%,手分离到的MRSA菌株qacA基因的阳性率为54.55%。结论上海市医院医护人员鼻前庭和手上MRSA携带率不高,但此类菌株抗消毒剂基因阳性率较高,提示应加强适时监控。
Objective To investigate the status of methicillin-resistant Staphylococccus aureus（MRSA） carrying and anti-resistance related genes in medical staff,and to provide reference for reasonable application of antibiotic in clinics.Methods Bacterial isolation and detection and Polymerase chain reaction（PCR） were used to detect the samples from noses and hands of medical staff.Results The carriage rate of methicillin-resistant Staphylococccus aureus of nasal was 1.81% and the carriage rate was 0.83% of medial staff hand of four different jobs in hospital.The carriage rate of doctor and nurse of were higher than others.The positive rate of qacA gene of nasal samples was 33.33% and the positive rate of qacA gene was 54.55% from hand carriage in the MRSA of medial staff.Conclusions The carriage rate of methicillin-resistant Staphylococccus aureus of nasal and medial staff hand in shanghai were low,but the postive rate of qacA was high,we should strengthen real-time monitoring.
出处
《中国消毒学杂志》
CAS
北大核心
2012年第7期565-568,共4页
Chinese Journal of Disinfection
基金
上海市卫生局"医院与社区获得性感染及医院环境中金黄色葡萄球菌耐消毒剂同源性研究"
编号2007021
关键词
耐氧西林金黄色葡萄球菌
携带
耐药基因
methicillin-resistant Staphylococccus aureus； carriage； drug-resistance gene
