摘要
背景:软骨细胞凋亡是骨关节炎(osteoarthritis,OA)发病过程中重要的病理学特征。YKL-40是壳质酶蛋白家族的一种糖蛋白,但不具有壳质酶活性,在关节炎软骨、滑膜、巨噬细胞等均有表达,可能与炎症的状态、组织重塑等功能有关。YKL-40在OA早中期中的作用尚有待研究。目的:探讨OA早中期关节软骨YKL-40表达与软骨细胞凋亡率(apoptosisindex,AI)的关系。方法:通过前交叉韧带切断术(anterior cruciate ligament transaction,ACLT)建立SD大鼠膝关节OA模型,组织学评估软骨退变程度,采用改良Mankin评分系统进行评估,免疫组织化学法检测YKL-40的表达情况及软骨细胞AI,观察两个指标的表达特点,分析两者在此病变过程中的关系。结果:随造模时间的延长,软骨出现退变并逐渐加重,YKL-40的表达与软骨细胞AI呈正相关。结论:软骨细胞凋亡是OA早中期的重要事件,YKL-40可能为OA早中期病理过程中软骨细胞凋亡的重要影响因子。
Background： Chondrocyte apoptosis is one of the main pathological characteristics of osteoarthritis. YKL-40 is a giycopro- tein of chitinase family, but the characterization of in vivo biological functions remains unknown. Previous studies showed that it may be related to tissue rebuilding and inflammation. YKL-40 expressed in several different cell types in the OAjoint tissue, including macrophages, articular chondrocytes and synoviocytes. The real role it plays in the OA pathogenesis needs to be investigated. Objective： The aim of the study is to explore the relationship between YKL-40 expression and chondrocyte apoptosis index （AI） in early and mediate stages of osteoarthritis. Methods： SD rat knee osteoarthritis model was made by anterior cruciate ligament transaction （ACLT）. The cartilages were evaluated by modified Mankin scoring. YKL-40 expression and chondrocyte AI were detected by immunohistochemy. The correlation of the YKL-40 expression and chondrocyte AI was analyzed by multiple regression calculation. Results： The severity of the cartilage degeneration aggravated along with the time of model establishment. The expression of YKL-40 was positively correlated to the apoptosis index of chondrocyte. Conclusions： Chondrocyte apoptosis is an important event at the beginning of osteoarthritis. YKL-40 may be an significant effective factor to the apoptosis during the pathological process.
出处
《中国骨与关节外科》
2012年第3期243-246,共4页
Chinese Journal of Bone and Joint Surgery
