摘要
目的研究大鼠臂丛根性撕脱伤后磷酸化CaMKⅡ的时间和空间定位。方法将SD大鼠(6～8周龄)在手术显微镜下做臂丛根性撕脱伤手术后,应用Western blot方法测定相应脊髓节段(C7、C8)磷酸化CaMKⅡ在1、2、3 d时间点及正常大鼠的表达水平。同时应用免疫荧光染色方法检测目标蛋白在1 d时间点大鼠脊髓节段的表达水平及空间定位。结果正常大鼠脊髓相应节段检测不到磷酸化CaMKⅡ,在臂丛根性撕脱伤后1～2 d对应脊髓节段的磷酸化CaMKⅡ表达明显升高,撕脱伤后3d明显下降并接近正常水平;免疫荧光结果显示:臂丛根性撕脱伤后1 d,损伤侧脊髓前角运动神经元和中间神经元明显表达磷酸化CaMKⅡ,对侧脊髓前角运动神经元和中间神经元也有表达,但强度较撕脱侧明显减弱。结论臂丛根性撕脱伤诱导磷酸化CaMKⅡ在损伤节段1 d时高表达,然而在3 d时逐步下降到正常水平。
Objectives To explore changes in spatial and temporal expression of phospho-CaMK Ⅱ in spinal cord section af- ter spinal root avulsion in adult rats. Methods SD rats （6 to 8 weeks） get brachial plexus root avulsion under the operating micro- scope. On 1 day, 2 day, 3 day after brachial plexus root avulsion and normal rats were set as control, phospho-CaMK Ⅱ were detect- ed in the pertinent spinal cord segments （C7, C8） by western blot. And immunofluorescence was used to find the location and the ex- pression of the CaMK Ⅱ in spinal cord section 1 day post injury. Results Phospho-CaMK Ⅱ can not be detected in normal rat spinal cord. Phospho-CaMK Ⅱ can only be detected within 3 days. Phospho-CAMK Ⅱ can be detected swiftly and reached its climax 24 hours after avulsion, then began to decrease. About three days after avulsion, phospho-CaMKⅡ can not be detected. Immunofluores- cence results showed that the phospho-CaMKⅡ can not be detected in normal rat spinal corrd but there is obvious CaMK Ⅱ expression in motoneurons and interneurons of the injuried side and less expression in the contraheral side of the spinal ventral horns in lesioned rats at 24 h post injury. Conclusions Phospho-CaMKⅡ could be induced at a high level lday after brachial plexus root avulsion in adult rats. However, it descents to normal level gradually within 3 days.
出处
《解剖学研究》
CAS
2012年第3期176-179,F0004,共5页
Anatomy Research
基金
国家自然科学基金项目(81070995
31171290)
