摘要
目的比较乙肝表面抗原-酶联免疫吸附试验一步法(简称一步法)和乙肝表面抗原-酶联免疫吸附试验二步法(简称二步法)检测乙肝表面抗原(HBsAg)的结果,研究钩状效应对一步法检测HBsAg结果的影响,评价二步法检测HBsAg的应用价值。方法分别用一步法和二步法检测1200份无偿献血者初检标本,并用一步法(稀释法)检测怀疑存在钩状效应的标本6份。结果一步法检测出29份HBsAg阳性,二步法检测出35份HBsAg阳性,6份一步法检测结果阴性怀疑存在钩状效应的标本进行1:5、1:10、1:30、1:60倍稀释后,再采用一步法进行检测,分别有6份、6份、4份、3份结果转为阳性。结论采用二步法或一步法同时对标本进行一定倍数稀释后检测,可以克服钩状效应,提高检测的敏感度。
Objective To compare the results of HBsAg measured by one-step and two-step ELISA,in- vestigate the influence of hook effect on HBsAg measure and to study the value of two-step ELISA in HBsAg measure. Methods A total of 1200 samples of unpaid blood donors＇ were tested by one-step HBsAg-ELISA and two-step HBsAg-ELISA separately. Six samples with suspected hook effect were measured with one-step ELISA （diluted）. Results A total of 29 cases were diagnosed positive by one-step ELISA, 35 eases were diag- nosed positive by two-step ELISA. After 1：5, 1：10, 1：30, 1：60 dilution. The six negative cases with suspected hook effect were detected again by one-step ELISA and 6, 6, 4, 3 cases turned to be positive respectively. Conclusion The hook effect can be eliminated by two-step ELISA or one-step ELISA after diluting all sam- ples and the detection sensitivity can be increased.
出处
《实验与检验医学》
CAS
2012年第3期269-270,共2页
Experimental and Laboratory Medicine
