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流式细胞仪计数造血干细胞的影响因素 被引量:5

Influencing factors of flow cytometry in enumerating CD34^+ cells
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摘要 造血干细胞计数对造血干细胞移植有重要意义。现临床上普遍使用流式细胞仪对送检标本中的造血干细胞进行计数,由于造血干细胞在标本中占单个核细胞的比例较低,流式细胞仪计数造血干细胞的影响因素较多,计数结果差异较大。为保证造血细胞计数的准确性和可比性,本文就影响因素做一综述,希望可以为实验室造血干细胞计数的质量保证工作提供帮助。 The enumeration of hematopoietic stem cells is important for hematopoietic stem cell transplantation. Us- ing flow cytometry to enumerate hematopoietic stem cells is widespread in clinical. Because the proportion of hematopoietic stem cells in mononuclear cells is very low, and there are many influencing factors of flow cy- tometry, the hematopoietic stem cell counts are quite different between laboratories. To improve the accuracy and comparability of the count, influencin~ f^e~tar~ ; n ;~ .L:
出处 《实验与检验医学》 CAS 2012年第3期213-215,218,共4页 Experimental and Laboratory Medicine
基金 科技部国家高科技研究发展计划863计划 项目编号:2011AA02A116
关键词 流式细胞术 造血干细胞 质量保证 Flow cytometry Hematopoietic stem cells Quality assurance
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参考文献19

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