摘要
目的观察鞘内注射选择性胶质细胞代谢抑制剂氟代柠檬酸对蜜蜂毒诱致的自发痛、痛觉过敏和炎症的作用。方法 40支鞘内置管的雄性SD大鼠随机分为单独蜜蜂毒注射组、溶媒对照组、低剂量(1nmol)氟代柠檬酸组、中剂量(10nmol)氟代柠檬酸组、高剂量(50nmol)氟代柠檬酸组(n=8)。注射前测定大鼠基础机械刺激阈值和鼠爪体积;鞘内注射后20min大鼠左后足底皮下注射蜜蜂毒(0.2mg),立即进行自发痛缩足反应观察,时程1h;2h后再次测定大鼠的机械刺激阈值和鼠爪体积。结果大鼠一侧后肢足底皮下注射蜜蜂毒可致持续1h的自发缩足反射,注射爪的机械刺激阈值降低,鼠爪体积增加。与对照组比较,鞘内预先注射氟代柠檬酸可明显抑制蜜蜂毒诱致的持续自发缩足反射和机械刺激阈值下降(P<0.05,P<0.01),且呈明显剂量依赖性(P<0.05),但对蜜蜂毒引起的鼠爪肿胀无明显影响。结论脊髓胶质细胞的激活可能参与了蜜蜂毒诱致的自发痛和痛觉过敏反应,但不参与炎症的发生。
Objective To observe the effects ofintrathecal injection of fluorocitrate, a glial metabolism inhibitor, on bee- toxin-induced spontaneous pain, hyperalgesia and inflammatory response. Methods Forty adult male SD rats with intrathecal catheterization were randomly divided into five groups （8 each）： （1） bee-toxin alone group; （2） vehicle （solvent） group; （3） low dose （lnmol） fluorocitrate group; （4） middle dose （10nmol） fluorocitrate group; （5） high dose （50nmol） fluorocitrate group. After the measurement of rat paw withdrawal mechanical threshold （PWMT） and paw volume （PV）, the drug or vehicle was administered intrathecally. Twenty minutes later, bee-toxin （0.2mg/50 ~ 1） was intraplantarly injected into the left hind paw of every rat, and spontaneous flinching reflexes （SFR） were observed instantly for 1 hour. Two hours later, PWMT and PV were measured again. Results Intrap｜antar iniection of bee-toxin into one hind paw of rat induced persistent SFK lasting for 1 hour, with PWMT decreased and PV increased in the injected paw. Compared with control group, pretreatment with intrathecal injection of fluorocitrate produced a significant inhibition of bee-toxin-induced persistent SFK （P〈0.05, P〈0.01）, decreased the PWMT in a dose-dependent manner （P〈0.05）, but it had no effect on bee-toxin-induced paw edema. Conclusion Activation of spinal glial cells may participate in bee-toxin-induced spontaneous pain and mechanical hyperalgesia, but not inflammatory response.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2012年第8期779-782,共4页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金(30471667,30772089)~~
关键词
注射
脊髓
神经胶质
疼痛
痛觉过敏
炎症
injections, spinal
neuroglia
pain
hyperalgesia
inflammation
