摘要
目的观察孕妇外周血和新生儿脐带血内皮祖细胞(endothelial progenitor cells,EPCs)数量和功能变化的相关性。方法用流式细胞仪检测未怀孕妇女和早孕妇女外周血中内皮祖细胞的数量;用密度梯度离心法分离脐带血内皮祖细胞;用流式细胞仪检测脐带血内皮祖细胞的数量;脐带血内皮祖细胞经体外培养7d后,分组做增殖实验、迁移实验和粘附实验。结果妇女怀孕后循环中内皮祖细胞数量增加;外周血内皮祖细胞数量明显降低的孕妇所产新生儿的脐带血内皮祖细胞数量降低,增殖、迁移和粘附能力下降,与对照组比较差异有统计学意义(P<0.05)。结论怀孕后循环内皮祖细胞数量增加明显;孕妇外周血和新生儿脐带血内皮祖细胞的数量和功能变化有一定的相关性。
Objective This study was conducted to analyze the number and functional change of endothelial progenitor cells（EPCs） in peripheral blood of normal women and umbilical cord blood. Methods Number of EPCs in peripheral blood of normal women and early pregnancy groups was analyzed by using flow eytometer. Umbilical EPCs were isolated by density gradient eeutrifugation. After culturing 7d, migration assay, proliferation assay and adhesive assay were executed. Results The number of endothelial progenitor cells increased after pregnancy. Number of umbilical cord blood EPCs decreased corresponding peripheral blood EPCs. At the same time, proliferation, migration and adhesion capacity decreased, There was significant difference between the experimental groups and the control group. Conclusion The numbers of EPCs increase obviously in peripheral blood of pregnant women. Maternal peripheral blood and umbilical cord blood EPCs number and function have some relevance.
出处
《中国计划生育和妇产科》
2012年第4期31-34,共4页
Chinese Journal of Family Planning & Gynecotokology
基金
山东省人口科技发展项目(200706)
关键词
内皮祖细胞
孕妇
外周血
脐带血
endothelial progenitor cells
pregnancy
peripheral blood
umbilical cord blood
