摘要
目的观察C26腺癌恶病质动物模型的自然发展过程。方法于BALB／C小鼠接种结肠腺癌Colon26（C26）细胞，在接种后0、5、10、15、20、25d，记录小鼠腓肠肌、附睾和去瘤体质量，检测生化指标、肿瘤坏死因子-α（TNF—α）和组织核因子-κB（NF—κB）及泛素蛋白酶体的E3连接酶（Atrogin）-l和肌肉环状指蛋白（MURF）-1基因的表达。结果本模型明显的营养不良和代谢紊乱发生在肿瘤接种后15d。荷瘤组F组较空白对照G组：白蛋白浓度分别为（14．44±1．12）、（19．80±2．45）g/L，（P〈0．01）；其他生化指标：TNF-α和NF—κB及泛素蛋白酶体的激活也有不同程度改善（P〈0．05）。结论癌症恶病质从起始发展到出现明显症状是一个较长的过程；血清白蛋白可以作为判断肿瘤患者是否发生恶病质的指标之一。
Objective To observe the natural developing process of the cancer cachexia-inducing C26 colon carcinoma in mice. Methods The murine colon 26 adenocarcinoma cells was inoculated subcu- taneously into BALB/C mice. On the day 0, 5, 10, 15, 20, and 25 after inoculation, body weight and gastrocnemius muscle with epididymal adipose were documented. Biochemical parameters, serum tumor necrosis factor-α （TNF-α）, the expression of nuclear factor-κB （NF-κB） in tumor and expression of ubiq- uitin proteasome E3 ligase （ Atrogin-1 ） and muscle ring finger protein 1 （ MURF-1 ） genese were valuated. Results Remarkable malnutrition and metabolic disorder occured about 15 days after the tumor inoculation in this model. There was significant difference in the concentrations of serum albumin between tumor-bear- ing group and healthy control group at the 15th day [ （ 14. 44 ± 1.12） vs （ 19. 80 ±2. 45） g/L,P 〈0. 01 ]. The other biochemical indicators, TNF-α, NF-κB, and Atrogin-1 and MuRF-1 had beneficial changes to varying degrees （P 〈 0. 05 ）. Conclusion Cancer cachexia development from the inoculated tumour ceils to the obvious symptoms is a long process. The serum albumin may serve as an indicator to judge cancer cachexia development.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2012年第7期1331-1334,共4页
Chinese Journal of Experimental Surgery
基金
福建医科大学教授学术发展基金资助项目（Js06045）
关键词
结肠腺癌
恶病质
模型
动物
Colon carcinoma
Cachexia
Model, animal
