摘要
目的观察红花甙(Carthamin)对模拟微重力(MMG)作用后人脐静脉内皮细胞(HUVECs)的增殖、迁移以及细胞骨架的影响,拟为多角度对抗心血管功能失调(CD)策略提供思路。方法 MMT摸索Carthamin作用最佳浓度,2D-clinostat进行失重模拟培养HU-VECs,分为NG对照组、MMG对照组、Carthamin+MMG组。采用细胞计数、流式细胞术检测各组细胞增殖情况,Transwell检测细胞迁移情况;观察Carthamin干预后细胞骨架蛋白的改变情况。结果 Carthamin的最佳给药浓度为10 mg/L。MMG可使HUVECs增殖明显抑制,但Carthamin的干预并没有逆转MMG导致HUVECs细胞增殖抑制的情况。Carthamin孵育48 h后,MMG组的迁移情况明显改善,MMG+Carthamin组较MMG对照组迁移细胞数增加了38%。Carthamin干预后,尽管弥散情况没有明显改善,但细胞的伪足较MMG对照组增多,微管改善不明显,MMG+Carthamin组仍然显示为弥散的数个微管亚聚体。结论 Carthamin可明显改善由于MMG作用而导致的HUVECs细胞迁移抑制,并修复由于MMG作用导致HUVECs细胞骨架f-actin的骨架重排现象,可能为中药干预CD提供新思路。
Objective It is to observe the interfering effect of Carthamin on proliferation, migration and cytoskeleton of HUVECs under MMG, thus to provide some thinking for anti-cardiovascular dysfunction from many ways. Methods The proper Carthamin concentration was gotten by MTT. HUVECs were cuhured under weightless simulation by 2D - clinorotat. All the samples were divided into 3 groups： NG control group, MMG control group and Carthamin ＋ MMG group. Cell counting and FCM was used to analyze cell proliferation. Transwell was used to detected cell migration. Confocal microscopy and image a- nalysis were used to analyze modifications of the cytokeratin network, and chromatin structure. Results The proper concentra- tion of Carthamin is 10 mg/L. HUVECs proliferation was inhibited in MMG. And it shows no signs of recovery when treated with Carthamin. The migration was improved when cells were incubated with Carthamin. Compared with MMG control group, the migrate cell number in MMG ＋ Carthamin group were increased 38% （P 〈 0.05 ） , while it still less than the NG controls. After incubated with Carthamin, the lamellipodia in MMG ＋ Carthamin cells were more plentiful than that in MMG control group although the f - actin still loose their typical radial array. Carthamin did not refine microtubules architecture the microtu- bules in MMG ＋ Carthamin group which still showed diffuse pattern. Conclusion Carthamin could repair the blocked HUVECs migration caused by MMG and it is greatly associative with refinement of f - actin structure, so this study may provide new idea for intervention for cardiovascular dysfunction with traditional Chinese medicine.
出处
《现代中西医结合杂志》
CAS
2012年第22期2410-2413,共4页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
国家自然科学基金资助项目(81102678)
关键词
模拟微重力
人脐静脉内皮细胞
红花甙
细胞骨架
迁移
modeled microgravity
human umbilical vein endothelial cells, Carthamin
cytoskeleton
migration
