摘要
利用谷氨酰胺转氨酶催化天然乳胶(NRL)水溶性蛋白质(WSP)发生交联,减少天然乳胶中水溶性蛋白质的含量。利用粒径分析、结构表征、十二烷基硫酸钠凝胶电泳实验证明了交联反应的发生。采用这一新技术可以降低天然乳胶制品的过敏性,拓展了其在医疗卫生领域、食品加工等领域的应用空间。
Water-soluble proteins existed in natural rubber latex can easily cause allergic reaction in people. Microbial Transglutaminase (MTGase)can modify latex proteins by means of covalent erosslinking to reduce the contewL of water-soluble proteins in natural rubber latex. The allergy pro- teins were considered to be catalysis of crosslinking ticle-size analysis,characterization of structure and allergic reaction of natural rubber latex products health,food processing and other fields. reaction with MTGase,which were verified by par- SDS-PAGE. Using this new technology can reduce and expand the application space in the area of health,food processing and other fields.
出处
《弹性体》
CAS
2012年第3期9-13,共5页
China Elastomerics
基金
上海市基础研究重点项目(09JC1404300)
关键词
天然乳胶
谷氨酰胺转氨酶
水溶性蛋白质
过敏
交联
natural rubber latex
microbial transglutaminase
water-soluble proteins
allergy
crosslinking