摘要
本实验中制备了诃子不同溶剂粗提物及部分纯化物,并考察了它们的清除自由基活性。1.1-二苯基-2-苦肼基(DPPH.)清除实验结果表明,所有粗提物均具有很强的活性,其顺序为:甲醇>乙酸乙酯>丙酮>乙醚>石油醚。其中甲醇粗提物(1 mg/mL)活性略高于等浓度的TBHQ;色谱纯样a比其原粗提取物(乙酸乙酯)的抑制率提高最多,为其1.6倍,而甲醇粗提物纯化样c的绝对活性最高,约为同浓度TBHQ(1 mg/mL)的1.2倍,且略高于茶多酚。它们的化学结构经显色反应、UV-Vis和H1NMR检测推定:a为诃黎勒酸,b为棓酸衍生物,是诃子单宁的小水解碎片,而c是诃黎勒酸的变体。
In this experiment the flee radical-scavenging activity of various components from terminalia chebula ritz was studied. The results showed that all extracts of terminalia chebula ritz stems had strong DPPH scavenging effects. The activity-decreased sequence of the extracts by different extracting regents was： methanol〉ethyl acetate〉acetone〉ether〉petro-ether. The methanol extract （lmg/mL） had better activity than that of TBHQ. The activities of the component A was 1.6 folds higher than the original. The chromatographically purified component C showed the strongest activity among all samples. Its activity was a little stronger than that of tea polyphenol, as 1.2 folds higher than that of TBHQ. By color test, UV-Vis and H^1 NMR analysis, all of the purified components were confirmed to be the derivatives of myrobalani-tannin or their hydrolyzed segments.
出处
《现代食品科技》
EI
CAS
北大核心
2012年第7期780-783,818,共5页
Modern Food Science and Technology
基金
平顶山学院高层次人才科研启动经费资助项目(2008013)
