摘要
目的:研究基于高效液相的黄连药性物质组群对实热证模型大鼠IL-1β、IL-6、IL-8、TNF-α含量的影响。方法:皮下注射2,4-二硝基苯酚复制大鼠实热证模型,分别使用黄连水煎液、热性趋势标记物、寒性趋势标记物,在模型复制后0.5h按体重(10ml/kg)灌胃进行治疗,测定IL-1β、IL-6、IL-8、TNF-α含量。结果:与空白对照组相比,模型对照组IL-1β、IL-6、IL-8、TNF-α含量明显升高(P<0.05);与模型对照组相比,水煎液组、寒性趋势标记组、IL-1β、IL-6、TNF-α含量明显降低(P<0.05),热性趋势标记组IL-6、IL-8、TNF-α含量明显降低(P<0.05)。结论:基于高效液相的黄连药性物质组群对实热证模型大鼠IL-1β、IL-6、IL-8、TNF-α含量升高有治疗作用。
Objective： To investigate the affects of berberine substance groups obtained from HPLC on the content of IL-1β,IL-6,IL-8 and TNF-α of the excessive heat syndrome rat model.Method： The excessive heat syndrome rat model was replicated by subcutaneous injection of 2,4-dinitrophenol.The serum content of IL-1β,IL-6,IL-8 and TNF-α was detected after the model was treat with hot trend markers,cold trend markers and the decotion group of berberine by gavage according to the weight of 10ml/Kg after the model copied 0.5 hours ago respectively.Result： Compared with the control group,the contend of IL-1β,IL-6,IL-8 and TNF-α increased significantly in the model group（P0.05）.At the same time,cold trends markers group and the decotion group＇s content of IL-1β,IL-6 and TNF-α and the hot trends markers group＇s content of IL-6,L-8 and TNF-α decrease significantly compared with model group（（P0.05）.Conclusion： The cold trend markers among the berberine substance groups obtained from HPLC have effects to cure the increasing metabolism of the excessive heat syndrome model rats.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2012年第1期130-131,共2页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家重点基础研究发展计划(973计划)资助课题(编号:2007CB512601)
