摘要
目的:探讨20(S)-人参皂苷-Rh2(GS-Rh2)对人膀胱癌T24细胞增殖和凋亡的影响及其可能的机制。方法:采用MTT法检测GS-Rh2对T24细胞增殖抑制率;光学显微镜观察GS-Rh2作用前后细胞形态变化;流式细胞仪检测GS-Rh2对T24细胞凋亡和细胞周期分布影响;RT-PCR检测mRNA表达。结果:GS-Rh2在5 mg/L～21.97mg/L浓度范围内,对T24细胞增殖无明显抑制作用;21.97 mg/L～160mg/L浓度范围内,GS-Rh2对人膀胱癌T24细胞的生长有抑制作用,呈量-效关系,IC50为37mg/L。T24细胞经GS-Rh2(40mg/L)作用后光镜下呈死亡形态改变;细胞增殖指数(PI)由42.534%下降至31.03%;早期凋亡细胞数明显增加;p53和myc mRNA表达下降。结论:GS-Rh2能显著抑制人膀胱癌T24细胞增殖、诱导T24细胞凋亡,其机制可能与GS-Rh2下调P53和myc mRNA表达,阻滞细胞进入S/G2期等有关。
Objective ：To observe the effect and mechanism of ginsenoside-Rh2（GS-Rh2）on proliferation and apoptosis of human bladder carcinoma T24 cells.Methods： Cell inhibition ratio（IR） of GS-Rh2 on T24 cells was evaluated by MTT colorimetric assay.Changes of morphology were observed by optical microscope after adding GS-Rh2 at different concentrations into the T24 cells.Apoptosis and cell cycles were examined with flow cytometry analysis.RT-PCR was used to detect the expressions of mRNA.Results： After the T24 cells dealt with GS-Rh2,MTT colormetric assay revealed that the inhibition ratios of 5 to 21.97 mg/L of Rh2 have no differences with the control.And the inhibition ratios of 21.97 to 160 mg/L of Rh2 on the growth of the T24 cells were in a dose-dependent manner,the 50% inhibition concertration（IC50） was 37.00mg/L.The typical apoptotic morphology was discovered after dealt with GS-Rh2（40mg/L）.The proliferation index（PI） dropped from 42.534% to 31.030%.And staining with Annexin V-FITC/PI showed that the early apoptotic cells increased evidently.RT-PCR revealed that the expressions of mutp53 and myc protein were down-regulated.Conclusions： GS-Rh2 exhibited growth inhibition effect and induced apoptosis on human bladder carcinoma T24 cells in vitro.The mechanism of it may be related with down-regulating mutp53,myc protein expression and arresting cell cycle into S/G2 phase.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2012年第1期35-38,共4页
Pharmacology and Clinics of Chinese Materia Medica
关键词
人参皂苷-Rh2
T24细胞
增殖
凋亡
Ginsenoside-Rh2（GS-Rh2）
human bladder carcinoma T24 cells
proliferation
apoptosis
