摘要
【目的】优选化橘红中肌醇最佳提取工艺。【方法】以乙醇浓度、料液比和提取温度为考察因素,采用高效液相—蒸发光散射检测器(HPLC-ELSD)色谱法测定化橘红中肌醇含量,采用正交试验法优选化橘红中肌醇最优提取工艺。【结果】化橘红中肌醇最佳提取工艺为体积分数70%乙醇、料液比1∶20、提取温度100℃、每次时间45 min、提取2次。【结论】本研究优选得到的提取工艺稳定、提取率高,可用于化橘红中肌醇的提取。
Objective To optimize the extraction technology for the inositol from Exocarpium Citri Grandis. Methods With ethanol concentration, the ratio of material to solvent and extracting temperature as factors, the extraction technology for the inositol from Exocarpium Citri Grandis was optimized by orthogonal design and through the determination of inositol content by high performance liquid chromatography-evaporative light scattering detector （HPLC-ELSD）. Results The optimal extraction technology was as follows： 70% ethanol, the ratio of material to solvent being 1： 20, extraction at 100 ~C for 45 min and extraction twice. Conclusion The extraction technology is steady, extraction percentage is higher and the technology is suitable for the extraction of inositol from Exocarpium Citri Grandis.
出处
《广州中医药大学学报》
CAS
北大核心
2012年第4期424-427,共4页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
国家科技部"十二五"科技支撑计划项目(编号:2011BAIOB02)
关键词
化橘红/化学
肌醇/分析
正交试验
提取工艺
色谱法
高压液相
EXOCARPIUM CITRI GRANDIS/chemistry
INOSITOL/analysis
ORTHOGONAL DESIGN
EXTRACTION TECHNOLOGY
CHROMATOGRAPHY, HIGH PRESSURE LIQUID PHASE
