摘要
目的比较生理盐水稀释法与ARCHITECT HBsAg试剂配套原液稀释法检测HBsAg定量的结果。方法留取HBsAg定量值超过250IU/ml的血清标本共96份,分别用ARCHITECT HBsAg试剂配套原液稀释(A法)、生理盐水稀释(B法)后进行HBsAg定量的检测,将91例均能得到具体测定值的样本纳入统计学分析,比较B法与A法检测结果的一致性。结果 A法、B法测定值的对数值范围分别为(2.72～4.92)lg IU/ml、(2.52～4.97)lg IU/ml,对数值的中位数分别是3.83lg IU/ml、3.81lg IU/ml。B法检测结果与A法检测结果的差异无统计学意义(P=0.712)。B法与A法检测结果的相关系数为0.969(P=0.000)。对检测结果采用Bland-Altman方法分析,结果显示,B法的检测结果与A法的检测结果具有很好的一致性,B法与A法检测结果的差值均数的绝对值接近0,一致性界限范围窄。在不同的HBsAg水平分层中,B法的检测结果与A法亦有很好的相关性。结论 B法的检测结果与A法的检测结果相关性高、差异程度低,具有很好的一致性。
Objective To compare two diluents in the HBsAg quantitative detection.Methods A total of 96 specimens with HBsAg over 250 IU/ml were included in the study.ARCHITECT HBsAg detection kit was used for further dilution analysis to compare two diluents：solution dilution in the kit（A method） and saline dilution（B method）.Results Data from 91 cases were included in the statistical analysis.The detection range of methods A and B were（2.72-4.92）and（2.52-4.97）lg IU/ml,respectively;the medians were 3.83 and 3.81 lg IU/ml,respectively.There was no significant difference between diluents A and B（P=0.712）.The correlation coefficient of the two methods was 0.969 and with high consistency by Bland-Altman analysis;the mean of difference between method A and B was close to 0.Methods A and B also showed very high correlation at different HBsAg levels.Conclusions Normal saline can be used as the diluents in the detection of HBsAg when using the ARCHITECT kit.
出处
《中国病毒病杂志》
CAS
2012年第4期267-270,共4页
Chinese Journal of Viral Diseases
基金
国家"十一五"艾滋病和病毒性肝炎等重大传染病防治科技重大专项(2009ZX10002-028)
关键词
肝炎表面抗原
乙型
稀释液
化学发光免疫法
Hepatitis B surface antigen
Diluent
Chemiluminescence immunoassay
