摘要
制备了电聚合硫堇膜修饰青霉素酶电极(青霉素酶/Thi/GCE),用于检测残留青霉素.运用循环伏安法研究了青霉素G钠在该电极上的电化学行为,并对电极的制作条件及检测条件进行了优化.结果表明在戊二醛质量分数为2.47%,牛血清蛋白质量分数为6.57%,固定化时间为2.44h,用酶量为50μL条件下制备的酶电极在pH为7.0,温度为25℃时响应性能达到最佳.该电极对青霉素G钠的线性范围为0.08～1.0μg/mL.
A penicillin enzyme electrode modified by the electrochemical polymerization of Thionine was prepared for the detection of penicillin residues. The electrochemical behavior of penicillin G sodium on the electrode was studied by using cyclic voltammetry, and the electrode manufacturing conditions and detection conditions were optimized . The results show that when the glutaraldehyde concentration is 2.47% ,the bovine serum albumin concentration is 6.57% ,the immobilization time is 2.44hours,and the volume of enzyme is 50μL. The prepared enzyme electrode will function best under the conditions when pH is 7.0, and temperature is 25 ℃. The linear rang of the electrode for detect penicillin G sodium is 0. 08 μg/mL-1. 0μg/mL.
出处
《安徽工程大学学报》
CAS
2012年第2期31-35,共5页
Journal of Anhui Polytechnic University
关键词
电聚合
青霉素酶
青霉素G钠
electrochemical polymerization
penicillin enzyme
penicillin G sodium
