摘要
目的研究外周血中miR-27a、miR-181b的表达水平在胃癌早期的诊断价值。方法采用RT-PCR法检测46例胃癌患者(A组)、27例胃癌前病变患者(B组)及21例健康人(C组)外周血浆中miR-27a、miR-181b的表达水平。结果与C组相比,A组和B组血浆中miR-27a、miR-181b的表达明显增加(P<0.01或P<0.05),ROC曲线下面积分别为0.876、0.770和0.769、0.749;而A组和B组miR-27a、miR-181b表达差异无统计学意义(P>0.05)。结论外周血浆中miR-27a、miR-181b有作为胃癌早期诊断标志物的潜力。
Objective To investigate the diagnostic value of expressions of miR-27a and miR- 181b in the peripheral blood for early detection of gastric cancer. Methods The expressions of miR- 27a and miR-181b were detected by RT-PCR in 46 cases of gastric cancer（group A）, 27 cases of gastric precancerous lesion（group B） and 21 cases of healthy people（group C）. Results Compared with group C, the expressions of miR-27a and miR-181b in plasma were obviously increased in groups of A and B（P〈0. 01 or P〈0. 05）. The area under ROC curve of miR-27a and miR-181b in group A was 0. 876 and 0. 770, which in group B was 0. 769 and 0. 749, respectively. Moreover, there was no significant difference of miR-27a and miR-181b between group A and group B（P〉0. 05）. Conclusion miR-27a and miR-181b may be taken as the potential biomarkers for early detection of gastric cancer.
出处
《江苏医药》
CAS
CSCD
北大核心
2012年第14期1665-1667,共3页
Jiangsu Medical Journal
关键词
胃癌
胃癌前病变
MIRNA
Gastric cancer
Gastric precancerous lesion
miRNA
