摘要
[目的]探讨暹罗斗鱼的发病原因和病害防治方法。[方法]对暹罗斗鱼的病原菌进行分离,并通过生理生化指标测定和16S rD-NA测序对其鉴定,同时进行药敏试验。[结果]菌株DY001为暹罗斗鱼嗜水气单胞菌病的病原菌。经鉴定,确定菌株DY001为嗜水气单胞菌(Aeromonas hydrophila)。该病原菌对暹罗斗鱼的半致死量(LD50)为0.04×106CFU/g。药敏试验结果表明,病原菌对阿奇霉素、奥复星、氟哌酸、复方新诺明、菌必治、甲氧苄啶和氯霉素等抗生素敏感。[结论]嗜水气单胞菌是引起此次暹罗斗鱼发病的病原菌,而药敏试验结果将为其防治提供参考。
[Objective] The research aimed to discuss the disease cause of Betta splenders Regan and the prevention and control methods of its diseases and pests.[Method] The pathogenic bacteria of B.splenders were isolated and identified by determining the physiological and biochemical indices and 16 S rDNA sequencing.And the drug sensitivity test was made.[Result] Strain DY001 was the pathogenic bacteria that caused Aeromonas hydrophila disease of B.splenders.Strain DY001 was identified as Aeromonas hydrophila.The median lethal dose（LD50） of this pathogeny to B.splenders was 0.04×10^6 CFU/g.The results of drug sensitivity test showed that this pathogenic bacteria was sensitive to azithromycin,ofloxacin,norfloxacin,compound sinomin,ceftriaxone,trimethoprim,chloramphenicol and other antibiotics.[Conclusion] Aeromonas hydrophila was the pathogenic bacteria that caused the disease incidence of B.splenders.The results of drug sensitivity test could provide references for the disease prevention and control of B.splenders.
出处
《安徽农业科学》
CAS
2012年第22期11289-11292,共4页
Journal of Anhui Agricultural Sciences
基金
海南省自然科学基金(311056)
海南三亚市重点科技项目(2010YF09)
