摘要
建立尿液和血清中培氟沙星药物残留的分析方法。利用Tb3+能增强Ce(Ⅳ)-SO32--PEFX体系的发光强度,建立测定培氟沙星的新方法。体系的发光强度与培氟沙星质量浓度在2×10-9～6×10-7g/mL范围内呈良好的线形关系,方法检出限为8.8×10-10g/mL,对1×10-7g/mL培氟沙星平行测定11次,相对标准偏差为3.1%。利用该法测定人尿样和鸭血清中培氟沙星,并进行回收率测定,回收率在98.5%～102.5%,结果令人满意。
An analytical method to determine pefloxacin （PEFX） residue in urine and serum was established based on the fact that the presence of Tb3~ enhances the luminous intensity of Ce（IV）-SO32-- PEFX system. There was a good linear relationship between the luminous intensity of the system and PEFX concentration over the range of 2×10-9-6×10-7g/mL, The limit of detection for PEFX was 8.8×10-10g/mL The relative standard deviation for 11 parallel determination of PEFX at 1×10-7g/mL was 3.1%. The average spike recoveries of PEFX from human urine and duck serum ranged from 98.5% to 102.5%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第12期215-218,共4页
Food Science
基金
韩山师范学院重点科研项目
关键词
流动注射化学发光法
培氟沙星
铽
尿样
血清
flow injection chemiluminescence
pefloxacin
terbium
urine
serum
