摘要
从兔脑下垂体中的ｍ ＲＮＡ中，利用合成引物逆转录ＰＣＲ，获得了前体和成熟生长激素的ｃＤＮＡ，成熟生长激素的ｃＤＮＡ克隆在ｐＥＴ３ａ 表达载体并转化到大肠杆菌ＢＬ２１（ＤＥ３ ｐｌｙｓ） 中，获得了高效表达，表达量达到大肠杆菌总蛋白的４０ ％ 。
The cDNA of preprotein and mature protein of rabbit hypophysiscerebri was obtained. The cDNA of mature growth hormone was cloned in pET\|3a, then the recombinant expression vector was transformed into E.coli BL21(DE3 plys).The overexpression was performed and the yield was 40% of the total protein.
出处
《北京大学学报（自然科学版）》
CAS
CSCD
北大核心
2000年第2期199-202,共4页
Acta Scientiarum Naturalium Universitatis Pekinensis
