摘要
为探寻提高粳型巨胚稻幼苗素质的方法,用PEG-6000配制成0,5%,10%,15%,20%,25%等6种不同质量浓度的溶液,对粳型巨胚稻西巨胚1号种子进行水分胁迫,用粳稻合系22-2(小胚)作对照,研究部分幼苗素质性状,试验方法不同,其结果差异较大:清水引发PEG液培养的水分胁迫处理不利于幼苗生长,随着PEG浓度的升高,幼苗生长势变弱,叶绿素和可溶性蛋白含量增加,根系活力下降,SOD活性在轻度、中度胁迫下上升,严重水分胁迫下呈下降趋势;PEG引发清水培养的水分胁迫处理在PEG质量浓度15%以下时对幼苗的生长有一定的促进作用,可溶性蛋白降低,SOD活性和根系活力升高,浓度高于15%后对幼苗生长、SOD活性和根系活力有抑制作用。西巨胚1号经5%～15%的PEG溶液引发24 h后再用清水培养的水分胁迫处理下幼苗素质有所提高。
To explore the giant embryos of Japonica rice to improve the quality of seedlings method, With conventional Japonica rice Hexi-22-2 as control, the experiment of water stress was conducted by using different concentrations （0, 5% , 10% , 15% , 20% , 25% ） of PEG-6000 with Japonica gi- ant embryo rice Xi-giant Embryo-1 to study the characters of seedling quality. The results indicated that different experimental methods made a great difference in results. The treatment that culture with different concentration PEG water after 24 hours water seed soaking were not conducive to seedling growth. With increasing concentration of PEG, the growth vigor of seedling became weak, and the con- tent of chlorophyll and soluble protein in seedling leaf was increased, but the root activity of seedling was decreased. The super oxide dimutese （SOD） activity was increased under light or moderate water stress and was downtrend under heavy water stress. Under the treatment that water culture after 24 hours seed soaking with the concentration PEG water below 15%, the germination and the growth of seedling and seedling formation were promoted, the soluble protein content in seedling leaf was de- creased, and the activity SOD root was increased; but the germination, the growth of seedling and seedling formation, the SOD activity and root activity were inhabited when higher concentration of PEG than 15%. The seedling of Japonica Giant Embryo Rice Xi-giant Embryo-! was enhanced under the treatment that water culture after 24 hours seed soaking with 5% -15% concentration PEG.
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第4期497-502,共6页
Journal of Yunnan Agricultural University:Natural Science
基金
四川省教育厅青年基金项目(2006C003)
关键词
粳型巨胚稻
水分胁迫
幼苗性状
Japonica giant embryo rice
water stress
seedling properties
