摘要
综述了目前主要核酸水解酶的种类、来源、蛋白质特性和生产方法;介绍了目前酶解法生产核苷酸的几种主要方法,比较了各种方法的优缺点及工业化应用前景;简单综述了水解液中四种核苷酸的分离提取方法;探讨了酶解法生产核苷酸的未来研究方向.结论为酶解法的关键是必须通过广泛采用现代高新生物技术来降低生产成本.
In this paper,the kinds、sources、protein characters and the manufacture methods of nucleic acid hydrolase were summarized;the mainly nucleotide production methods with the hydrolase were indroduced,and the advantages、defects、industry prospects of these methods were also be comparised;the separation and extraction methods of four single nucleotides in the hydrolyzed liquid were simply summarized;the future studies trends of nucleotides production by enzyme method were also be discussed.The Conclusion was that the enzyme methods must lowered the production cost through the widely using of modern biotechnology.
出处
《辽宁大学学报（自然科学版）》
CAS
2012年第2期110-117,共8页
Journal of Liaoning University：Natural Sciences Edition
基金
2010年科技部农业科技成果转化资金项目"核苷酸类畜禽益生保健制剂生产技术中试与示范"
2008年科技部农业科技成果转化资金项目"生态高效规范化淡水养殖关键技术示范"(2008GB2C100110)
农业部淡水鱼类遗传育种和养殖生物学重点开放实验室开放基金项目"新型安全高效水产饲料添加剂--酵母核苷酸的研究开发"(BZ2009)
关键词
核酸
核苷酸
磷酸二酯酶
核酸酶P1
固定化
超滤
盐析
纯化
nucleic acid
nucleotide
phosphodiesterase
nucleaseP1
immobilization
ultrafiltration
salting out
purification
