摘要
为了进行小豆种质创新和获得基因定位和克隆及其基因功能分析的突变体材料,分别用30、35、40、45、50、55Gy剂量的快中子,辐照小豆‘京农6号’干种子。M1出苗率分别为31.72%、29.06%、30.29%、36.51%、16.15%和15.17%,变异率为3.20%、4.70%、6.75%、6.61%、4.00%和2.20%。辐射剂量增加,出苗率降低、变异率呈增加趋势,M2代共获得77份叶形、株型、粒型、籽粒大小、粒色、叶色、早熟、丰产等性状变异材料。40～45Gy为小豆快中子诱变的适宜剂量,这些突变体为小豆相关基因定位克隆、功能分析和育种提供了有价值的材料。
The aim was to obtain the mutant materials of azuki bean tbr gene location, clone and gene functional analysis. Dry seeds of azuki bean variety ＇Jingnong 6＇ were irradiated with 30, 35, 40, 45, 50, 55 Gy dosage fast neutron respectively for creating new germplasm and obtaining mutants of gene mapping, cloning and functional analysis. Germination rates of M, generation were 31.72%, 29.06%, 30.29%, 36.51%, 16.15% and 15.17% respectively. Their relative variation frequencies were 3.20%, ＇$.70%, 6.75%, 6.61%, 4.00%, 2.20%. The higher dosage of fast neutrons was, the lower germination rate was, and the higher variation frequence was relative. The dosages from 40 Gy to 45 Gy were optimistic to azuki bean fast neutron mutagenesis. Seventy seven mutants including leaf shape, plant architecture, seed shape and size, seed coat color, leaf color, early maturity and high yield in M2 generation were gained. These mutants were very useful for gene mapping, gene cloning, further functional analysis and breeding.
出处
《中国农学通报》
CSCD
2012年第18期53-58,共6页
Chinese Agricultural Science Bulletin
基金
北京市教委科研计划面上项"铁吸收关键基因LeFRO1在小豆中的转化利用"(KM201010020004)
北京市教2011科基-科创平台项目"国家自然科学基金‘小豆高密度分子遗传图谱构建’项目匹配"(5075227014)
关键词
小豆
快中子诱变
突变体
azuki bean （ Vigna anguladsi）
fast neutron mutagenesis
mutant
