摘要
目的检测REV3基因低表达后对COLO-205细胞增殖的影响情况。方法采用RNA干扰技术特异性的下调人类结肠癌细胞(COLO-205)中REV3基因的表达情况:实验组细胞加入的培养基中包含脂质体及降低REV3基因表达的质粒,阴性对照组细胞加入的培养基中包含脂质体及空质粒,空白对照组细胞只加入培养基。实时荧光定量PCR技术检测不同细胞REV3基因的相对表达量,通过细胞计数和MTT检测,比较REV3基因表达量不同的细胞生长增殖情况。结果 REV3基因的表达量降低后,COLO-205实验组细胞的增殖速度与对照组细胞相比较明显降低,差别有统计学意义(P<0.05);而阴性对照组和空白对照组的细胞增殖情况虽然有一定的差异,但差别没有统计学意义。结论特异性的下调REV3基因在COLO-205中的表达量,可能对该细胞的生长与增殖均具有一定的抑制作用。
Objective To detect the effect of decreasing REV3 gene expression on COLO - 205 cell proliferation state. Methods RNA interference technology was used to down -regulate the REV3 gene expression in COLO -205 cells. Plasmid with down -regulation REV3 gene was transfected with liposome transfection rea- gent. plasmid without down- regulation REV3 gene was transfeeted into cells as negative control group, cells without any treatment were used as blank control group. Use qRT - PCR to detect the REV3 gene expression in different cell group and compare the cell proliferation state at different REV3 gene expression level by cell number counting and MTT test, Results Cell proliferation condition was significantly decreased when REV3 gene expression down -regulated, which compared to the other two groups （P 〈0.05）. No significant differ- ence was found between negative control group and blank control group （ P 〉 0.05 ）. Conclusion Down - regulated REV3 gene expression in COLO -205 cells may inhibit the cell growth and proliferation, but more work is needed to investigate the possibility.
出处
《宁夏医科大学学报》
2012年第6期572-575,F0003,共5页
Journal of Ningxia Medical University
基金
宁夏自然科学基金(NZ0885)
宁夏医科大学校级科研项目(XQ201002)
