摘要
【目的】探讨生长激素(GH)基因对新疆褐牛早期生长性能的影响,为新疆褐牛选育提高奠定分子遗传基础。【方法】以116头份新疆褐牛血样为研究对象,利用PCR-RFLP技术检测GH基因第5外显子上AluⅠ突变位点(GH/AluⅠ位点)的多态性,并分析不同基因型与新疆褐牛早期生长性状的相关性。【结果】GH/AluⅠ位点在新疆褐牛群体中表现为VV、LV和LL3种基因型,其基因频率分别为0.138、0.397和0.465;新疆褐牛GH/AluⅠ位点的杂合度为0.4408,多态信息含量为0.3437;不同基因型与早期生长发育性状的关系为:1～4月龄的LV和LL基因型个体体重、体长均较VV基因型个体的优,且差异显著(P<0.05),而出生体重、5~6月龄体重、体长差异不显著(P>0.05)。【结论】GH/AluⅠ位点的L等位基因对新疆褐牛早期生长发育性状有正向选择作用,可作为新疆褐牛辅助选择的遗传标记。
[ Objective ]Effects of GH gene on early growth traits of Xinjiang Brown Cattle were investigatool in order to provide molecular genetic information for breeding Xinjiang Brown Cattle. [Method]Blood samples of total 116 Xinjiang Brown Cattle were collected to detect polymorphism of Alu Ⅰ mutational site on 5^th exon of GH using PCR-RFLP method. Correlation between genotype polymorphism and early growth traits was also assayed. [Result]GH/Alu Ⅰ site was expressed in Xinjiang Brown Cattle as VV, LV, LL genetype with gene frequencies 0.138, 0.397 and 0.465, respectively. Heterozygosity and polymorphic information of GH/Alu I were 0.4408 and 0.3437, respectively. Relations between various genotypes and early growth traits were as follows： for 1-4 months old cattle, body weight and length of LV and LL genotypes were superior to VV genotype, and the difference was significant （P〈0.05）. However, no significant differences were observed in VV, LV, LL genotypes for birth weight and body weight and length of 5-6 months old cattle. [Conclusion]Owing to its positive role in early growth traits, L allele of GH gene can be selected as an assistant-selected genetic marker for Xiniiang Brown Cattle.
出处
《南方农业学报》
CAS
CSCD
北大核心
2012年第5期688-691,共4页
Journal of Southern Agriculture
基金
新疆重大科技专项项目(200731135)
