摘要
目的探讨大鼠慢性酒精中毒后大脑皮质、海马、小脑的病理学改变及caspase-3的异常表达。方法选用健康雄性Wistar大鼠随机分为两组,其中酒精中毒组30只;盐水对照组20只。酒精中毒组每日每只大鼠分别按8ml/kg灌胃2w,随后再按照10ml/kg灌胃1w,按12ml/kg灌胃1w,共灌胃4w。每日灌胃两次,其间隔均为6h,酒精浓度为50%。对照组用等量的生理盐水灌胃。并对两组大鼠进行体重、一般生物学特征、HE染色、TUNEL染色、免疫组化caspase-3的检测。结果造模成功后,两组大鼠的体重存在的统计学差异;HE染色后酒精组大鼠大脑皮质、海马、小脑锥体细胞数目减少,部分神经元变性、坏死;TUNEL法测定酒精组大鼠凋亡细胞数量明显多于对照组(P<0.05),酒精组大鼠大脑皮质、海马、小脑的caspase-3表达明显高于对照组(P<0.05)。结论慢性酒精中毒可引起大鼠大脑皮质、海马及小脑的病理学改变,出现神经细胞凋亡,引起与凋亡相对应部位caspase-3阳性表达,并参与大鼠酒精中毒后凋亡机制的发生、发展。
Objective To discuse the change of pathology and expression of caspase-3 in cerebral cortex,hippocam- pus and cerebellum of alcoholism rats. Methods There were 50 male healthy Wistar rats divided into 2 groups randomly, alcoholism group ,30 rats, saline control group ,20 rats. Alcoholic group ： every rat was fed with 8ml/kg 50% alcohol twice a day, and two weeks later, increased to 10ml/kg for one week, then 12ml/kg for one week. The interval of time was 6 hours of all. Control group ： every rat was fed with the same dosage of 0.9% sodium chloride at the same time for four weeks. During the experiment, we measured their weight, observed their general condition, HE dyes, TUNEL dying and expression of caspase-3 by SP dying method. Results After 4 weeks, the alcoholic group rats appeared malnutrition, emaciated, moreo- ver, some also appeared the performance of nervous system damages. The cell in cerebral cortex, hippocampus and cerebel- lum pyramidal of alcoholic group had diminuted in number, and the nerve cell had arranged disorder by HE dyes medthod. Apoptosis ceils of alcoholic group increased obviously compare to control group by TUNEL method （ P 〈 0.05 ）. Alcoholic groups increased caspase-3 expression by SP dying method （ P 〈 0.05 ）. Conclusion The chronic alcoholism may cause the rats cerehral cortex, hippocampus and cerebellum＇s pathology change and nerve cell apoptosis and caspase-3 mRNA mas- culine expression with the corresponds spot of apoptosis.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2012年第6期516-519,共4页
Journal of Apoplexy and Nervous Diseases
基金
吉林省卫生厅科研课题(No.20082028)
