摘要
目的:鉴定人前列腺癌雄激素依赖性细胞株LNCaP、前列腺癌雄激素非依赖性细胞株PC-3与良性前列腺增生细胞株BPH-1之间差异表达的核基质蛋白(nuclear matrix proteins,NMPs)。方法:常规制备各细胞株NMPs,应用双向凝胶电泳对其进行分离;对差异表达的蛋白质点行MALDI-TOF-MS/MS质谱分析,数据库搜索并鉴定。结果:成功获得了分辨率高、重复性好的不同细胞株NMPs双向凝胶电泳图谱;初步鉴定出包含酶、调节蛋白、RNA结合蛋白及各种因子在内的12个差异表达的蛋白质,在癌细胞株中3个NMPs表达上调,9个下调。结论:人前列腺癌细胞与良性前列腺增生细胞之间NMPs表达存在明显差异;初步筛选出12个差异NMPs,其表达水平及功能与疾病的联系需进一步研究。
Objective： To compare the expression of nuclear matrix proteins （NMPs） in benign prostatic hyperplasia （ BPH） epithelial cell line BPH-1 versus those in androgen-dependent human prostate cancer cell line LNCap and androgen-independent prostate cancer cell line PC-3. Methods ： We isolated NMPs from the BPH-1, LNCap and PC-3 cell lines by 2-dimensional electrophoresis （2-DE）, analyzed the differentially expressed proteins by matrix-assisted laser desorption / ionization time of flight mass sPectrometry （MALDI-TOF-MS）, and identified them by peptide mass fingerprint and database searching. Results： We successfully obtained well-resolved reproducible 2-DE patterns of NMPs in human prostate cancer cell lines, identified 12 differentially expressed NMPs ineluding enzymes, regulatory proteins, RNA-binding protein and various other factors, 3 up-regulated and 9 down-regulated in prostate cancer cell lines. Conclusion： There are obvious differences in the expressions of NMPs between human prostate cancer cell lines and benign prostatic hyperplasia epithelial cell line.
出处
《中华男科学杂志》
CAS
CSCD
2012年第7期583-589,共7页
National Journal of Andrology
基金
中央级公益科研院所基本科研业务费专项(2009GJSSJKB07)~~
