摘要
31只健康豚鼠分为哮喘组、地塞米松干预组及对照组。测定各组豚鼠肺泡灌洗液细胞成分 ,应用RT PCR方法检测支气管组织白细胞介素 5(IL 5)和白细胞介素 1 0 (IL 1 0 )mRNA表达强度。结果显示 ,哮喘组豚鼠肺泡灌洗液中嗜酸性粒细胞 (EOS)百分比、IL 5mRNA表达量均显著高于地塞米松治疗组及对照组 ,而IL 1 0mRNA表达则相反。IL 5mRNA表达与EOS百分比呈正相关 ,而与IL 1 0mRNA表达呈负相关。说明EOS ,IL 5以正相关参与了哮喘发病。哮喘时IL 1 0表达受抑制 ,这可能是炎症细胞因子IL 5合成增多的主要原因之一。糖皮质激素治疗哮喘的作用机制之一 ,可能与其提高IL 1 0及下调IL
To explore the expression of interleukin 5(IL 5) and interleukin 10(IL 10) and their relationship in asthma, we measured the cells in bronchial aveolar lavage fluid(BALF) of guinea pigs, and examined the expression of IL 5 mRNA and IL 10 mRNA of bronchial tissue by reverse transcription polymerase chain reaction(RT PCR) in control group(Group C, n=10), asthmatic group(Group A, n=11) and dexamethasone treatment group(Group B, n=10). The results showed that the eosinophil(EOS) percentage and the expression of IL 5 mRNA were significantly higher in Group A[(48.64±17.8)%, (106.91±20.09)% respectively] than that in Group C[(15.10±11.48)%, (62.60±13.84)%] and Group B[(28.4±10.32)%, (75.30±10.70)%]. In constrast, the IL 10 mRNA expression in Group A[(47.5±5.3)%] was obviously lower compared with that in Group C[(101.2±4.2)%] and Group B[(80.1±8.3)%]. The correlation between the expression level of IL 5 mRNA and percentage of EOS was positive(r=0.924 P<0.01), whereas the IL 5 mRNA was negatively correlated with IL 10 mRNA expression(r=-0.731, P<0.01). Conclussion: IL 5 and EOS might play a role in the pathogenesis of asthma, the inhibition of IL 10 mRNA expression may cause the increase of IL 5 expression in asthma. The benefical effects of corticosteroid treatment in asthma may partly result from increasing IL 10 mRNA expression and inhibiting IL 5 mRNA expression. [
出处
《湖南医科大学学报》
CSCD
2000年第2期99-102,共4页
Bulletin of Hunan Medical University
基金
CMB资助项目! (82 4 1 0 )
关键词
哮喘
白细胞介素5
白细胞介素10
基因表达
bronchial asthma
interleukin -5
interleukin -10
eosinophils
reverse transcription polymerase chain reaction
