摘要
对诱变红酵母RY-09菌株生物合成类胡萝卜素的培养基组成和培养条件进行初步探索.通过生长曲线的测定和各种发酵条件的优化,确定最佳培养条件:初始pH值4.5、培养温度26℃、培养时间64 h;在最优条件下,红酵母生物量为9.03 g·100 mL-1、菌体生物合成类胡萝卜素最大量为5.46 mg·L-1.同时采用HPLC法初步确定类胡萝卜素的主要组成.
The paper deals with the culture medium compositions and fermentation conditions of biosynthesis of carotenoids from rhodotorula RY-09 strain.Through the determination of the growth curve and the optimization of fermentation conditions,some main parameters were selected,with the initial pH at 4.5,the temperature at 26 ℃ and the fermentation time set for 64 hours.The composition of carotenoids was mostly detected by HPLC.The cell of rhodotorula RY-09 and the content of carotenoids were 9.03 g/100 mL and 5.46 mg·L-1 respectively.
出处
《南京晓庄学院学报》
2012年第3期76-79,共4页
Journal of Nanjing Xiaozhuang University
基金
江苏省科技支撑计划资助项目(BE2010675)
关键词
红酵母
类胡萝卜素
培养条件
Rhodotorula sp.
carotenoid
culture conditions
