摘要
目的了解介质表面污染的肠道病毒71型（EV71）在不同温、湿度条件下的存活能力。方法将滴度为1×10^5半数组织培养感染剂量（TCID50）的EV71株均匀滴加在灭菌的塑料、橡胶、木块和布片介质表面，置人不同温、湿度气候条件的人工气候箱中，一定时间后回收介质表面病毒，回收液接种Vero细胞，观察致细胞病变效应（CPE）；每日监测不同气候条件下介质表面病毒存活情况。结果病毒在4种介质表面回收率为89％～93％；暴露在不同温、湿度条件下介质表面的EV71存活时间不同，在20℃、湿度80％的气候条件下病毒存活时间最长；塑料表面的病毒滴度在24h后下降lgTCID50值约为4，布片和木块表面的病毒滴度在72h后下降lgTCID50值约为4．5，72h后至少下降lgTCID50值为3．89。结论介质表面污染EV71存活时间受温、湿度气候条件影响，模拟的4种气候条件中，温度低、湿度高的条件病毒存活时间长；EV71在布片、木块表面的存活能力强于合成材质塑料表面。
Objective To evaluate the survival ability of enterovirus 71 （EV71） on different surface and under different climate. Methods Each 1×10^5 tissue culture infective dose 50 （TCID50） EV71 was added on different aseptic surface of plastic, rubber, cloth and wood, respectively. Then these materials were put into biotron （artificial climatic chamber） which could simulate different temperature and moisture. The viruses were recovered after a definite time and then inoculated into Veto cell. The cytopathic effect （CPE） was observed everyday to survey the survival ability of EV71 on different medium surface. Results The recovery rates of EV71 on medium surface ranged from 89 %- 93 %. The survival time of EV71 on medium surface varied under different climatic conditions. The longest survival time of the virus was observed under the condition of 20 as the temperature and 80% as the humidity. After 24 hours of incubation, the infectious titer on plastic surface reduced about 4 lg. After 72 hours of incubation, the infectious titer reduced at least 3.89 lg on cloth and wood surface. Conclusions Temperature and humidity can affect the survival time of EVT1 on medium surface, which is longer in the condition of low temperature and high humidity. The survival ability of EV71 on natural cloth and wood surface is better than that on synthetic plastic surface.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2012年第7期398-401,共4页
Chinese Journal of Infectious Diseases
基金
上海市重点学科建设资助项目（B118）
关键词
手足口病
肠道病毒A型
人
存活率
湿度
温度
Hand, foot and mouth disease
Enterovirus A, human
Survival rate
Humidity
Temperature
