摘要
目的研究酒精性慢性胰腺炎组织中白细胞分化抗原14(CD14)、钟样受体4(TLR4)、肿瘤坏死因子(TNFα)的表达,探讨酒精性慢性胰腺炎的发病机制。方法 24只1月龄雄性SD大鼠随机分为对照组、脂多糖组、酒精组、酒精联合脂多糖组(以下简称联合组)各6只。酒精组和联合组饲以25%酒精,饮酒12周后联合组和脂多糖组,反复腹腔注射脂多糖2mg/kg·w,共4次。用免疫组化及RT-PCR检测CD14、TLR4、TNF在各组的表达。结果酒精组CD14、TLR4和TNF表达较对照组和脂多糖组增加(P<0.05),联合组CD14、TLR4和TNF表达较对照组、脂多糖组明显增加(P<0.01),较酒精组增加(P<0.05)。结论酒精性慢性胰腺炎组织中CD14、TLR4和TNF表达增加,脂多糖通路可能参与了慢性胰腺炎发生发展。
Objective To study the expression of leukocyte differentiation antigen 14 （ CD14 ）, toll-like receptor 4 （TLR4） and tumor necrosis factor α （TNF） in alcohol-induced chronic pancreatitis tissues,in order to investigate the potential mechanism of pathogenesis of alcohol-in- duced chronic panereatitis. Methods Twenty-four one-month old male SD rats were randomly divided into 4 groups ： control group, alcohol group,Lipopolysaccha rides （LPS） group, and alcohol-combined lipopolysaceharides group （combined group）. Rats in alcohol group and combined group were fed with 25% alcohol. After twelve weeks, the rats in combined group and LPS group were intraperitoneally injected with LPS （2 mg/kg） , once a week, for 4 times. Immunohistochemistry and RT-PCR were employed to observe the expression of CD14, TLR4, TNF in pancreas in each group during the experiment. Results Compared with control and LPS group, CD14, TLR4, TNF were highly ex- pressed in alcohol group （P 〈 0.05 ）. Compared with control and LPS group, CD14 ,TLR4 ,TNF were significantly highly expressed in com- bined group （ P 〈 0. 01 ）, Compared with al-cohol group, CD14, TLR4, TNF were strong expressed in combined group （ P 〈 0. 05 ）. Conclu- sion CD14 ,TLR4 ,TNF strong express in alcohol group, CD14, TLR4, TNF significantly strong express in combined group, which indicating that the signaling pathway of LPS may be involved in the development of alcohol-induced chronic pancreatitis.
出处
《世界科技研究与发展》
CSCD
2012年第3期478-481,共4页
World Sci-Tech R&D
基金
重庆市卫生局科研项目(062096)
重庆市自然科学基金(CSTC
2010BB5424)资助
