摘要
抗坏血酸过氧化物酶(Ascorbate peroxidase,APX)是H2O2清除剂,在植物抗逆反应中起着重要作用。根据NCBI发布的过氧化物体抗坏血酸过氧化物酶(Peroxisomal APX,pAPX)基因序列设计简并引物,分别以青花菜(Brassica oleracea var.italica)叶片DNA和cDNA为材料进行PCR扩增,克隆到一个APX基因,命名为BoAPX2,序列提交GenBank,登录号为JN172929。BoAPX2的基因组序列长为2 013 bp,具有8个内含子,编码区全长864 bp,编码287个氨基酸。序列分析结果表明,BoAPX2与已知的过氧化物体APX有较高的相似性,氨基酸的C端具1个跨膜结构域。RT-PCR结果表明,BoAPX2的表达受霜霉病菌(Hyaloperonospora parasitica)、H2O2、水杨酸和NaCl诱导。
Ascorbate peroxidase （APX）, a scavenger of hydrogen peroxide, plays an important role in plant stress resistance. Degenerate primers were designed according to peroxisomal APX sequences published in NCBI. APX gene, designated BoAPX2, was isolated from DNA and cDNA of leaves of Brassica oleracea var. italica, respectively. Gene sequence was submitted to GenBank with an accession number of JN172929. The full length genomic DNA of BoAPX2 was 2 013 bp in length with 8 introns, and the complete coding sequence was 864 bp in length encoding 287 amino acids. Sequence analysis results indicated that BoAPX2 shared high homology to known peroxisomal APXs and was possessed of a transmembrance domain at its C-terminal. RT- PCR results showed that the expression of BoAPX2 was induced by Hyaloperonospora parasitica, H202, salicylic acid and NaCl.
出处
《植物病理学报》
CAS
CSCD
北大核心
2012年第4期374-380,共7页
Acta Phytopathologica Sinica
基金
浙江省自然科学基金项目(Y3080081)
台州市科技计划项目(08XH02)
关键词
青花菜
霜霉病
抗坏血酸过氧化物酶
克隆
表达
Brassica oleracea var. italica
downy mildew
ascorbate peroxidase
cloning
expression
