摘要
目的 研究不同缺血方式对大鼠肾脏组织中三磷酸腺苷 (ATP)水平的影响。方法 将大鼠分为 4组 :假手术组 (A组 ) ;持续缺血组 (B组 ) ;间断缺血组 (C组 ) ;缺血预处理组 (D组 )。利用生物化学发光法测量不同缺血 再灌注处理后大鼠肾组织ATP水平的变化。结果 各缺血组大鼠肾脏在缺血终末期时ATP水平明显下降 ,仅为正常对照组的 1 0 %左右。再灌注 30min后 ,C组肾组织ATP水平恢复到正常水平的 60 %左右 ,而B组、D组肾组织ATP水平无明显恢复。结论 间断缺血有利于在短期内恢复因组织缺血造成的ATP水平的下降 ,从而减轻细胞损伤。
Objective\ To study the effects of different ischemic ways on ATP level in the rat kidneys. Methods\ Male Wistar rat kidneys were applied to sham group (group A), continuous ischemia group (group B), intermittent ischemia group (group C) and ischemic preconditioning group (group D). The levels of ATP in rat kidneys undergoing different ischemia reperfusion treatments were determined by using chemiluminescence assay.Results\ The level of ATP in each ischemic group was decreased significantly at the end stage of ischemia and about 10% of sham group ( P <0.01). After reperfusion for 30 min, the ATP level in the group C returned to about 60% of the normal level, still had no obvious recovery in group B and group D ( P <0.001). Conclusion\ Intermittent ischemia is beneficial to recover the decreased tissue ATP level caused by ischemia during a short period and alleviate cell injury. The ATP level of the kidney which suffered from ischemia for a long time will be hard to recover.\;
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2000年第3期219-220,共2页
Chinese Journal of Experimental Surgery
