摘要
目的:探讨脂肪酸结合蛋白(I-FABP)2基因编码区exon2第54位点的多态性与2型糖尿病(DM)发病风险的相关性。方法:采用随机化同期平行病例-对照试验分子流行病学研究方法,2型DM患者117例,正常对照组108例。应用聚合酶链反应(PCR)技术检测225例样本I-FABP2基因Hae-II酶切位点的多态性。结果:(1)样本人群的I-FABP2基因编码区exon2存在Hae-II酶切位点,可产生多态性片段:野生型I-FABP2Ala54/Ala54;变异型I-FABP2Ala54/Thr54;Thr54/Thr54。(2)在DM组变异型I-FABP2Ala54/Thr54及Thr54/Thr54基因型频率显著高于正常对照组(P<0.05)。(3)I-FABP2 Ala54/Thr54;Thr54/Thr54基因携带者比野生型基因携带者发生2型DM的风险OR值为1.97(95%CI,1.13～3.44)。(4)I-FABP2基因54位密码子的变异频率在男性及女性中近似相等(P>0.05)。结论:I-FABP2基因编码区exon2第54位点的多态性与2型DM发病风险的相关;携带I-FABP2基因变异型Ala54/Thr54;Thr54/Thr54的个体增加发生2型DM的风险。
Objective：To investigate the fatty acid binding protein（I-FABP） 2 gene coding region of exon2 54 points polymorphism with type 2 diabetes（DM） risk.Methods：Randomized parallel cases over the same period-the control and experimental molecular epidemiology research methods,117 cases of type 2 DM patients and normal control group of 108 cases.Polymerase chain reaction（PCR） detected 225 cases of samples of I-FABP2 gene Hae-II restriction site polymorphism.Results：（1） The sample population of the I-FABP2 gene coding region exon2,the existence Hae-II restriction enzyme sites can produce polymorphic fragments： wild-type I-FABP2Ala54/ Ala54;variant I-FABP2Ala54/Thr54;Thr54/Thr54.（2） In the DM group the variant I-FABP2Ala54/Thr54 and Thr54/Thr54 genotype frequency was significantly higher than the normal control group（P〈0.05）.（3） Of I-FABP2 Ala54/Thr54;Thr54/Thr54 gene carriers than wild-type gene carriers,the risk of type 2 DM OR value of 1.97（95% CI,1.13 to 3.44）.（4） The I-of I-FABP2 gene 54 codon mutation frequency in males and females are approximately equal（P〉0.05）.Conclusion：The gene encoding the the exon2 first 54,I-of I-FABP2 polymorphism and type 2 DM risk;Carry the I-of I-FABP2 gene variant Ala54/Thr54;Thr54/Thr54 the individual increases the occurrence of the risk of type 2 DM.
出处
《中国医药导刊》
2012年第6期930-931,933,共3页
Chinese Journal of Medicinal Guide
