摘要
本实验采用雄性S.D.大鼠,经日一次灌注2.2ml/kg 四氯化碳,于染毒后12、24、48小时测定肝微粒体Ca^2+-ATP 酶和肝细胞液磷酸化酶a 活性变化。结果发现,染毒后大鼠肝微粒体Ca^2+-ATP 酶活性显著降低,以染毒后24小时为甚;肝细胞液磷酸化酶a 活性显著增高,以染毒后24小时为最高。说明四氯化碳致肝损伤时,肝内质网隔离钙的能力降低,胞液中钙浓度升高。本实验对于阐明四氯化碳中毒性肝病发病机理有一定的意义。
Carbon tetrachloride was administered to male Sprague-Dawley rats in asingle hepatotoxic dose of 2.2ml/kg.The activity of liver-microsomalCa^(2+)-ATPase and liver-cytosol phosphorylase a was measured at 13,24 and48hr postadministration.The results were that the activity of Ca^(2+)-ATPasewas remsrkably decreased,while of phosphorylase a was highly increased.These data demonstrated that the ability of hepatic endoplasmic reticulum tosequester calcium is destroyed during CCl_4 poisoning.causing calcium to ac-cumulate in liver-cytosol.This experiment has certain significance in revealingthe mechanism of hepatosis induced by CCl_4 poisoning.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
1990年第4期215-217,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
关键词
四氯化碳中毒
肝脏
磷酸化酶
Ca^(2+)-ATPase
Phosphorylase a
CCI_4 poisoning Ca^(2+) homeostasis
